フルテキストファイル
著者
Muraoka Kuniyasu Department of Urology and First Department of Pathology, Tottori University Faculty of Medicine
キーワード
apoptosis
renal function
testosterone
抄録
The effect of testosterone replacement in middle-aged rats on renal function and apoptosis of mesangial and renal tubule cells was evaluated. Middle-aged (13-month-old) male Wistar rats were divided into 2 groups, one of which was given high-dose and the other low-dose testosterone replacement for 2 months. Experiments were performed with the testosterone replacement groups and a non-replacement group when they had reached the age of 15 months. Serum levels of total and free testosterone were measured, and renal function was evaluated by glomerular filtration rate (GFR), serum levels of creatinine, blood urea nitrogen (BUN), urinary protein and N-acetyl-beta-d-glucosaminidase (NAG). Apoptosis was evaluated by counting the number of terminal deoxynucleotidyl transferase-mediated deUTP-biotin nick end labeling (TUNEL)-positive cells in the mesangial and renal tubule cells. Correlations between total and free testosterone and each parameter were evaluated. Also, 12-week-old rats were evaluated in the same manner as the age control. Among 15-month-old rats, testosterone replacement did not affect serum creatinine, BUN or urinary protein, but the GFR was found to correlate negatively with the total and free testosterone level, and the urinary NAG level was found to correlate positively with the free testosterone level to a significant extent (P < 0.01). Percentages of apoptosis of mesangial and renal tubule cells were found to correlate ,positively with the total and free testosterone levels to a significant extent (P < 0.01). In conclusion, the results of this study suggested that testosterone replacement affected the deterioration of renal function in middle-aged rats.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
44
1
開始ページ
37
終了ページ
44
発行日
2001-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2001, 44(1), 37-44
部局名
医学部・医学系研究科・医学部附属病院
言語
英語