フルテキストファイル
著者
稲葉 央 Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University 研究者総覧 KAKEN
Nagata, Miyuu Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Miyake, Kyeongmi Juliano Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Kabir, Arif Md. Rashedul Faculty of Science, Hokkaido University
Kakugo, Akira Faculty of Science, Hokkaido University / Graduate School of Chemical Sciences and Engineering, Hokkaido University
Sada, Kazuki Faculty of Science, Hokkaido University / Graduate School of Chemical Sciences and Engineering, Hokkaido University
松浦 和則 Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University 研究者総覧 KAKEN
抄録
The cyclization of peptides is a valuable strategy for the development of binding motifs to target proteins with improved affinity. Microtubules (MTs) are important targets for therapeutics, and a variety of MT-targeted drugs and peptides have recently been developed. We have previously designed a Tau-derived peptide (TP) that binds to the interior of MTs. In the present study, the development of a cyclic TP (TCP) for enhanced binding to tubulin and the stabilization of MTs is described. The fluorescently labeled cyclic peptide containing three glycine linkers (TCP3-TMR) exhibited a remarkably enhanced binding affinity to tubulin. The cyclic peptide was also demonstrated to stabilize MTs by enhancing polymerization and reducing depolymerization. Moreover, MTs were effectively formed by the treatment of cyclic peptides in the presence of guanosine triphosphate (GTP), while the linear peptide showed no such effect. These findings indicate that TCP is a useful binding motif that can stabilize MTs and is valuable for various therapeutic and material applications.
出版者
Springer Nature
資料タイプ
学術雑誌論文
外部リンク
ISSN
00323896
EISSN
13490540
掲載誌名
POLYMER JOURNAL
52
9
開始ページ
1143
終了ページ
1151
発行日
2020-09
出版者DOI
著者版フラグ
著者版
著作権表記
(C) 2020 Springer Nature. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1038/s41428-020-0356-3
掲載情報
Inaba Hiroshi, Nagata Miyuu, Miyake Kyeongmi Juliano, et al. Cyclic Tau-derived peptides for stabilization of microtubules. POLYMER JOURNAL. 2020. 52(9). 1143-1151. doi:10.1038/s41428-020-0356-3
部局名
工学部・工学研究科
言語
英語
Web of Science Key ut
WOS:000532635300002