フルテキストファイル
著者
Yamaguchi Shigeyuki Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University
Niwa Ryosuke Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University
Kazuki Yasuhiro Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Chromosome Engineering Research Center, Tottori University 研究者総覧 KAKEN
Ohbayashi Tetsuya Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University 研究者総覧 KAKEN
キーワード
bacterial artificial chromosome
bacterial artificial chromosome recombineering
human artificial chromosome
抄録
Exactly controlled conditional gene expressing systems are crucial for genomic functional research, animal transgenesis and gene therapy. Bacterial artificial chromosomes (BACs) are optimal for harboring long fragments of genomic DNA or large cDNA up to 300 kb in size. Therefore, BACs are available to produce transgenic cells and animals for the functional studies of genes. However, BAC can insert DNA randomly into the host genome, possibly causing unpredicted expression. We previously developed a human artificial chromosome (HAC) vector from human chromosome 21 using chromosome engineering. The HAC vector has several important characteristics desired for an ideal gene delivery vector, including stable episomal maintenance, and the ability to carry large genomic DNA containing its own regulatory element, thus allowing physiological regulation of the transgene in a manner similar to that of the native chromosome. In this study, we develop a system fusing BAC library and HAC technology together to allow tight control of gene expression. This system enables BAC to be cloned into the defined locus on the HAC vector by the Cre/loxP system. In addition, the genome in the BAC is possible to be engineered freely by the BAC recombineering technology. This system is a highly efficient tool for the rapid generation of stringently controlled gene expression system on the HAC vector.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
外部リンク
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
54
1
開始ページ
21
終了ページ
31
発行日
2011-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2011, 54(1), 21-31
言語
英語