フルテキストファイル
著者
Nakamoto Sachiko Department of Medical Technology, Tottori University, Institute of Medical Care Technology KAKEN
キーワード
Candida albicans
corn meal broth
germ tube formation
non-slip slide glass incubation method
抄録
Candida albicans IFO 1385 yeast cells dominantly develop germ tubes in a dilute corn meal broth at 37°C. For germ tube (GT) development the broth concentration is optimal at 75% of the original, and the inoculum is optimal at 105 cells/mL. Incubation procedures were compared and the non-slip slide glass incubation (NSSI) method was found to be more available than the tube incubation method or slid-covered slide glass incubation method. The GT formation was completed in 1 to 2 h in the NSSI method and clear-cut GTs were observed under optical microscopy. GTs were formed in all 111 test-strains of C. albicans but not at all in other species of Candida. Therefore, under optimal conditions, the rate of identifying C. albicans is 100%, demonstrating that GT detection with the NSSI method is a rapid and reliable identification method. The humid NSSI method is suited for continuous observation of a GT. The initially formed GT elongated to resemble a true hypha, attaching no chlamydoconidium. The hypha subsequently dissociated yeast-like cells.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
41
2
開始ページ
65
終了ページ
72
発行日
1998-07
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 1998, 41(2), 65-72
部局名
医学部・医学系研究科・医学部附属病院
言語
英語