フルテキストファイル
著者
藪田 行哲 Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University 研究者総覧 KAKEN
Nagata, Ryuta Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Aoki, Yuka Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Kariya, Ayumi Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Wada, Kousuke Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Yanagimoto, Ayako Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Hara, Hiroka Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
美藤 友博 Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University 研究者総覧 KAKEN
Okamoto, Naho The United Graduate School of Agricultural Sciences, Tottori University
Yoshida, Shinichi Electronic and Organic Material Laboratory, Tottori Institute of Industrial Technology
石原 亨 Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University 研究者総覧 KAKEN
渡邉 文雄 Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University 研究者総覧 KAKEN
キーワード
L-ascorbate (AsA)
antioxidant
biosynthetic pathway
Caenorhabditis elegans
reactive oxygen species
redox
抄録
Ascorbate (AsA) is required as a cofactor and is widely distributed in plants and animals. Recently, it has been suggested that the nematode Caenorhabditis elegans also synthesizes AsA. However, its biosynthetic pathway is still unknown. To further understand AsA biosynthesis in C. elegans, we analyzed the incorporation of the 13C atom into AsA using gas chromatography-mass spectrometry (GC-MS) in worms fed with D-Glc (1-13C)-labeled Escherichia coli. GC-MS analysis revealed that AsA biosynthesis in C. elegans, similarly to that in mammalian systems, involves carbon skeleton rearrangement. The addition of L-gulono-1,4-lactone, an AsA precursor in the mammalian pathway, significantly increased AsA level in C. elegans, whereas the addition of L-galactono-1,4-lactone, an AsA precursor in the plant and Euglena pathway, did not affect AsA level. The suppression of E03H4.3 (an ortholog of gluconolactonase) or the deficiency of F54D5.12 (an ortholog of L-gulono-1,4-lactone oxidase) significantly decreased AsA level in C. elegans. Although N2- and AsA-deficient F54D5.12 knockout mutant worm (tm6671) morphologies and the ratio of collagen to non-collagen protein did not show any significant differences, the mutant worms exhibited increased malondialdehyde levels and reduced lifespan compared with the N2 worms. In conclusion, our findings indicate that the AsA biosynthetic pathway is similar in C. elegans and mammals.
出版者
MDPI
資料タイプ
学術雑誌論文
外部リンク
EISSN
22181989
掲載誌名
METABOLITES
10
8
開始ページ
334
発行日
2020-08
出版者DOI
著者版フラグ
出版社版
著作権表記
(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
掲載情報
Yabuta Yukinori, Nagata Ryuta, Aoki Yuka, et al. L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans. METABOLITES. 2020. 10(8). doi:10.3390/metabo10080334
部局名
農学部・農学研究科
言語
英語
Web of Science Key ut
WOS:000564136300001