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Izawa, Hironori Graduate School of Engineering, Tottori University
Okuda, Noriko Graduate School of Engineering, Tottori University
Yonemura, Tomoe Graduate School of Engineering, Tottori University
Kuroda, Kohei Department of Veterinary Clinical Medicine, Tottori University
Ochi, Kosuke Department of Veterinary Clinical Medicine, Tottori University
Morimoto, Minoru Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University 研究者総覧 KAKEN
Noda, Mayuko Department of Veterinary Clinical Medicine, Tottori University
cell culture substrate
Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 °C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress.
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
IZAWA, Hironori, et al. Application of bio-based wrinkled surfaces as cell culture scaffolds. Colloids and Interfaces, 2018, 2.2: 15.