フルテキストファイル
著者
Fujiwara, Kazunori Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine 研究者総覧 KAKEN
Yazama, Hiroaki Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine 研究者総覧 KAKEN
Donishi, Ryohei Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine
Koyama, Satoshi Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine 研究者総覧
Fukuhara, Takahiro Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine 研究者総覧 KAKEN
Kitatani, Kazuyuki Laboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University
Kataoka, Hideyuki Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine 研究者総覧 KAKEN
Takeuchi, Hiromi Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine 研究者総覧 KAKEN
抄録
Background: Anaplastic thyroid carcinoma (ATC) is an aggressive type of thyroid cancer, and its metastasis requires cell motility. Ceramide is involved in a variety of biological processes, including inflammation, cell signaling, cell motility, and induction of apoptosis, however has not previously been reported to inhibit the motility of ATC cells. We evaluated the effect of short chain C6-ceramide on motility of ATC cells.

Methods: Cell motility of 8305C thyroid carcinoma cell line treated with C6-ceramide was assessed using a transwell migration assay and a pseudopodia formation assay.

Results: Treatment with 10 μM C6-ceramide resulted in significantly fewer migratory cells than control treatment in a transwell migration assay (P < 0.002). In condition medium, 82.6% of C6-ceramide–treated cells formed lamellipodia. Importantly, treatment with 10
μM C6-ceramide drastically decreased the number of cells forming lamellipodia by 17.6% (P < 0.01).

Conclusion: Our results suggest that treatment with a low concentration of ceramide may prevent metastasis and recurrence of ATC by inhibiting cell motility. Further studies are necessary to investigate the mechanism of inhibition of cell motility by ceramide. Ceramide shows promise as a therapeutic treatment for ATC.
出版者
Tottori University Medical Press
資料タイプ
学術雑誌論文
外部リンク
ISSN
05135710
書誌ID
AA00892882
掲載誌名
Yonago Acta Medica
最新掲載誌名
Yonago Acta Medica
63
2
開始ページ
95
終了ページ
98
発行日
2020-05-18
出版者DOI
著者版フラグ
出版社版
著作権表記
注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.
掲載情報
K. Fujiwara, H. Yazama, R. Donishi, et al. C6-ceramide Inhibits the Motility of Anaplastic Thyroid Carcinoma Cells. Yonago Acta Medica. 2020, 63(2), 95-98. doi:10.33160/yam.2020.05.001
部局名
医学部・医学系研究科・医学部附属病院
言語
英語