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A Scanning Electron Microscopic Study of the Basal Surface of the Corneal Endothelium and the Stromal and Endothelial Surfaces of Descemet's Membrane in Rats Sasaki, Keiko 10032327 Tamai, Akihiko Tamai, Akihiko 20116312 Inoué, Takao Inoué, Takao Sasaki, Keiko Yonago Acta medica　編集委員会 corneal endothelium Descemet's membrane non-coating observation method sputter-coating with platinum corneal endothelium Descemet's membrane non-coating observation method sputter-coating with platinum The basal surface of the corneal endothelium and the stromal and endothelial surfaces of Descemet's membrane in rats were studied by scanning electron microscopy. We compared the fine structures of the two surfaces of Descemet's membrane both after sputter-coating with platinum and without sputter-coating. Fine structures were made clearly visible without metal coating by heating specimens to 300°C during observation. After sputter-coating, both surfaces of Descemet's membrane were composed of granular substances, but their sizes were larger on the stromal side than on the endothelial side. Both surfaces of Descemet's membrane observed without sputter-coating were composed of fine fibrous structures showing a felt-like appearance, but their diameters were thicker on the stromal surface. These results may reflect a difference in collagen types between the two surfaces of Descemet's membrane. Tottori University Faculty of Medicine 2004-03 eng journal article VoR https://repository.lib.tottori-u.ac.jp/records/5090 Yonago Acta medica. 2004, 47(2), 29-35 AA00892882 13468049 Yonago Acta medica Yonago Acta medica 47 2 29 35 https://repository.lib.tottori-u.ac.jp/record/5090/files/47_029-035.pdf application/pdf 491.3 kB 2010-01-20