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Exploration of Genes Associated with Sponge Silicon Biomineralization in the Whole Genome Sequence of the Hexactinellid Euplectella curvistellata. 90326877 清水, 克彦 シミズ, カツヒコ Shimizu, Katsuhiko 20776421 美藤, 友博 ビトウ, トモヒロ Bito, Tomohiro 80393411 有馬, 二朗 アリマ, ジロウ Arima, Jiro Kobayashi, Hiroki Nishi, Michika Tsukahara, Masatoshi (C) The Author(s) 2018. This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medi biosilicification Silica Silicatein Glassin Chitin biosilicification Silica Silicatein Glassin Chitin Silicatein is the first protein isolated from the silicon biominerals and characterized as constituent of the axial filament in the silica spicules of the demosponge Tethya aurantia, by significant sequence similarity with cathepsin L, an animal lysosomal protease, and as a catalyst of silica polycondensation at neutral pH and room temperature. This protein was then identified in a wide range of the class Demospongiae and in some species of the class Hexactinellida. Our attempt to isolate silicatein from the silica skeleton of Euplectella was unsuccessful, but instead we discovered glassin, a protein directing acceleration of silica polycondensation and sharing no significant relationship with any proteins including silicatein. The present study aims to verify the existence of silicatein by exploring the whole genome DNA sequence database of E. curvistellata with the sequence similarity search. Although we identified the sequences of glassin, cathepsin L and chitin synthetase, an enzyme synthesizing chitin, which has already been found in the silicon biominerals in E. aspergillum, silicatein failed to be identified. Our result indicates that silicatein is not essential for poriferan silicon biomineralization in the presence of glassin. Springer 2018-10-10 eng book VoR https://repository.lib.tottori-u.ac.jp/records/7063 9789811310010 9789811310027 Shimizu, K., Kobayashi, H., Nishi, M., Tsukahara, M., Bito, T., Arima, J. (2018). Exploration of Genes Associated with Sponge Silicon Biomineralization in the Whole Genome Sequence of the Hexactinellid Euplectella curvistellata . In: Endo, K., Kogure, T., https://link.springer.com/chapter/10.1007/978-981-13-1002-7_16 https://link.springer.com/chapter/10.1007/978-981-13-1002-7_16 https://repository.lib.tottori-u.ac.jp/record/7063/files/bm_147.pdf application/pdf 240.8 kB 2023-03-17