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Smooth muscle cell sheet transplantation preserve cardiac function and minimize cardiac remodeling in a rat myocardial infarction model 80529420 原田, 真吾 ハラダ, シンゴ Harada, Shingo 80335538 中村, 嘉伸 ナカムラ, ヨシノブ Nakamura, Yoshinobu 20601933 白谷, 卓 シラヤ, スグル Shiraya, Suguru 藤原, 義和 フジワラ, ヨシカズ Fujiwara, Yoshikazu 20623911 岸本, 祐一郎 キシモト, ユウイチロウ Kishimoto, Yuichiro 大月, 優貴 オツキ, ユキ Otsuki, Yuki 60714532 岸本, 諭 キシモト, サトル Kishimoto, Satoru 20362882 山本, 康孝 ヤマモト, ヤスタカ Yamamoto, Yasutaka 60211504 久留, 一郎 ヒサトメ, イチロ Hisatome, Ichiro 90291442 西村, 元延 ニシムラ, モトノブ Nishimura, Motonobu Onohara, Takeshi © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in an Cell sheet Smooth muscle cells Myocardial infarction Cell survival Cell sheet Smooth muscle cells Myocardial infarction Cell survival Background: We examined whether a vascular smooth muscle cell (SMC) sheet is effective in the treatment of a rat myocardial infarction (MI) model. Methods: We examined the effect of SMC sheet on the cardiac function and cardiac remodeling in a rat MI model in comparison with their effect of dermal fibroblast (DFB) sheet in vivo. Furthermore, we estimated the apoptosis and secretion of angiogenic factor of SMC under hypoxic condition in comparison with DFB. Seven days after MI, monolayer cell sheets were transplanted on the infarcted area (SMC transplantation group, SMC-Tx; DFB transplantation group, DFB-Tx; no cell sheet transplantation group, Untreated; neither MI nor cell sheet transplantation group, Sham). We evaluated cardiac function by echocardiogram, degree of cardiac remodeling by histological examination, and secretion of angiogenic growth factor by enzyme immunoassay. Results: Twenty-eight days after transplantation, SMC-Tx showed the following characteristics compared with the other groups: 1) significantly greater fractional area shortening (SMC-Tx, 32.3 ± 2.1 %; DFB-Tx, 23.3 ± 2.1 %; untreated, 25.1 ± 2.6 %), 2) suppressed left ventricular dilation, smaller scar expansion, and preserved wall thickness of the area at risk and the posterior wall, 3) decreased fibrosis, preserved myocardium in the scar area, and greater number of arterioles in border-zone, 4) tight attachment of SMC sheets on the scarred myocardium, and less apoptotic cell death. In in vitro experiments, SMCs secreted higher amounts of basic fibroblast growth factor (SMC, 157.7 ± 6.4 pg/ml; DFB, 3.1 ± 1.0 pg/ml), and showed less apoptotic cell death under hypoxia. Conclusions: Our results illustrate that transplantation of SMC sheets inhibited the progression of cardiac remodeling and improve cardiac function. These beneficial effects may be due to superior SMC survival. BioMed Central Ltd. 2016-08-05 eng journal article VoR https://repository.lib.tottori-u.ac.jp/records/7247 10.1186/s13019-016-0508-x Harada Shingo, Nakamura Yoshinobu, Shiraya Suguru, et al. Smooth muscle cell sheet transplantation preserve cardiac function and minimize cardiac remodeling in a rat myocardial infarction model. JOURNAL OF CARDIOTHORACIC SURGERY. 2016. 11. doi:10.1186/s13 https://cardiothoracicsurgery.biomedcentral.com/articles/10.1186/s13019-016-0508-x https://cardiothoracicsurgery.biomedcentral.com/articles/10.1186/s13019-016-0508-x 17498090 JOURNAL OF CARDIOTHORACIC SURGERY JOURNAL OF CARDIOTHORACIC SURGERY 11 https://repository.lib.tottori-u.ac.jp/record/7247/files/jcs11_s13019-016-0508-x.pdf application/pdf 1.7 MB 2023-03-17