2026-03-08T20:36:28Z https://repository.lib.tottori-u.ac.jp/oai

oai:repository.lib.tottori-u.ac.jp:00004724 2023-09-28T23:52:13Z 1:9 2:12 23:34:988:990

C6-ceramide Inhibits the Motility of Anaplastic Thyroid Carcinoma Cells 90403419 Fujiwara, Kazunori 30444631 Yazama, Hiroaki Donishi, Ryohei Koyama, Satoshi 80403418 Fukuhara, Takahiro Kitatani, Kazuyuki 00224436 Kataoka, Hideyuki 40197273 Takeuchi, Hiromi Yonago Acta medica　編集委員会 Background: Anaplastic thyroid carcinoma (ATC) is an aggressive type of thyroid cancer, and its metastasis requires cell motility. Ceramide is involved in a variety of biological processes, including inflammation, cell signaling, cell motility, and induction of apoptosis, however has not previously been reported to inhibit the motility of ATC cells. We evaluated the effect of short chain C6-ceramide on motility of ATC cells. Methods: Cell motility of 8305C thyroid carcinoma cell line treated with C6-ceramide was assessed using a transwell migration assay and a pseudopodia formation assay. Results: Treatment with 10 μM C6-ceramide resulted in significantly fewer migratory cells than control treatment in a transwell migration assay (P < 0.002). In condition medium, 82.6% of C6-ceramide?treated cells formed lamellipodia. Importantly, treatment with 10 μM C6-ceramide drastically decreased the number of cells forming lamellipodia by 17.6% (P < 0.01). Conclusion: Our results suggest that treatment with a low concentration of ceramide may prevent metastasis and recurrence of ATC by inhibiting cell motility. Further studies are necessary to investigate the mechanism of inhibition of cell motility by ceramide. Ceramide shows promise as a therapeutic treatment for ATC. Tottori University Medical Press 2020-05-18 eng journal article VoR https://repository.lib.tottori-u.ac.jp/records/4724 10.33160/yam.2020.05.001 K. Fujiwara, H. Yazama, R. Donishi, et al. C6-ceramide Inhibits the Motility of Anaplastic Thyroid Carcinoma Cells. Yonago Acta Medica. 2020, 63(2), 95-98. doi:10.33160/yam.2020.05.001 https://www.jstage.jst.go.jp/article/yam/63/2/63_2020.05.001/_article/ https://www.jstage.jst.go.jp/article/yam/63/2/63_2020.05.001/_article/ http://www.lib.tottori-u.ac.jp/yam/yam/yam63-2/63-2contents.html http://www.lib.tottori-u.ac.jp/yam/yam/yam63-2/63-2contents.html AA00892882 05135710 Yonago Acta Medica Yonago Acta Medica 63 2 95 98 https://repository.lib.tottori-u.ac.jp/record/4724/files/yam63(2)_95.pdf application/pdf 2.9 MB 2023-03-15