{"created":"2024-02-21T05:16:39.993695+00:00","id":2001320,"links":{},"metadata":{"_buckets":{"deposit":"871e8b92-674f-4f0e-9b01-73749e478472"},"_deposit":{"created_by":10,"id":"2001320","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2001320"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:02001320","sets":["1:10","2:12"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-09-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicNumberOfPages":"7","bibliographicPageEnd":"977","bibliographicPageStart":"971","bibliographicVolumeNumber":"95","bibliographic_titles":[{"bibliographic_title":"Pure and Applied Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Glycosphingolipids (GSLs) in the mammalian plasma membrane are essential for various biological events that occur on cell membranes by forming glycolipid-rich membrane domains, such as lipid rafts. Lactosylceramide (LacCer) forms a highly ordered phase in model and biological membranes. However, the details of this domain remain unclear. We examined the dynamic assembly of LacCer in cholesterol-containing phase-separated membranes by fluorescence imaging and solid-state NMR. Solid-state 2H NMR of the deuterated LacCer probes in the membranes indicated that the potent LacCer–LacCer interaction significantly reduced the incorporation of cholesterol in the LacCer domains. Moreover, increasing the amount of cholesterol induced the formation of nanometer-scale LacCer domains, which can be biologically relevant. The lateral interaction of ganglioside GM3 with the epidermal growth factor receptor (EGFR) transmembrane segment was examined using fluorescence spectroscopy under membrane conditions. Lateral FRET between the NBD fluorophore on the EGFR transmembrane segment and the ATTO594 fluorophore on the GM3 head group suggested that GM3 interacts with the transmembrane segment and partially inhibits the EGFR dimerization by stabilizing the peptide monomer. More precise investigations of GSL–GSL and GSL-protein interactions occurring in membrane environments can lead to the elucidation of the cellular functions of GSL from structural points of view.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"IUPAC & De Gruyter","subitem_publisher_language":"en"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Hanashima, Shinya, Nakano, Mikito and Murata, Michio. \"Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes\" Pure and Applied Chemistry, vol. 95, no. 9, 2023, pp. 971-977. https://doi.org/10.1515/pac-2023-0204"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.1515/pac-2023-0204"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1515/pac-2023-0204","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2023 IUPAC & De Gruyter","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00334545","subitem_source_identifier_type":"PISSN"}]},"item_10001_text_31":{"attribute_name":"Web of Science Key ut","attribute_value_mlt":[{"subitem_text_value":"WOS:000980113000001"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Graduate School of Science, Osaka University /  Graduate School of Engineering, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Graduate School of Science, Osaka University"},{"subitem_text_language":"en","subitem_text_value":"Michio Murata, Graduate School of Science, Osaka University / ForeFront Research Center, Osaka University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"embargoed access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_f1cf"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"15101","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"鳥取大学","affiliationNameLang":"ja"},{"affiliationName":"Tottori University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"花島,慎弥","creatorNameLang":"ja"},{"creatorName":"Hanashima,Shinya","creatorNameLang":"en"},{"creatorName":"ハナシマ,シンヤ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"花島","familyNameLang":"ja"},{"familyName":"Hanashima","familyNameLang":"en"},{"familyName":"ハナシマ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"慎弥","givenNameLang":"ja"},{"givenName":"Shinya","givenNameLang":"en"},{"givenName":"シンヤ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"100002461","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100002461_ja.html"},{"nameIdentifier":"50373353","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50373353"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Nakano,Mikito","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nakano","familyNameLang":"en"}],"givenNames":[{"givenName":"Mikito","givenNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Osaka University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Murata,Michio","creatorNameLang":"en"}],"familyNames":[{"familyName":"Murata","familyNameLang":"en"}],"givenNames":[{"givenName":"Michio","givenNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-09-25"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2023-09-26"}],"filename":"pac95(9)_971.pdf","format":"application/pdf","licensefree":"(C) 2023 IUPAC & De Gruyter","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"pac95(9)_971.pdf","objectType":"fulltext","url":"https://repository.lib.tottori-u.ac.jp/record/2001320/files/pac95(9)_971.pdf"},"version_id":"e24d4605-efca-40e0-a0b8-3f425b32a5f2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bilayer membranes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescence spectroscopy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glycosphingolipids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"interaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ICS-30","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["10","12"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-02-21"},"publish_date":"2024-02-21","publish_status":"0","recid":"2001320","relation_version_is_last":true,"title":["Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-02-22T00:09:06.939903+00:00"}