{"created":"2025-01-22T00:06:05.919275+00:00","id":2001684,"links":{},"metadata":{"_buckets":{"deposit":"139bf266-498c-40e5-b9ed-9a2e89ac6cd4"},"_deposit":{"created_by":10,"id":"2001684","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2001684"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:02001684","sets":["1:9","2:12","23:34:1710471377862:1737507731107"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-11-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicNumberOfPages":"9","bibliographicPageEnd":"292","bibliographicPageStart":"284","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Yonago Acta Medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background Pseudomonas aeruginosa is an important causative agent of bacterial keratitis. This study investigates the antibacterial effect of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS504 (TONS504-PACT) and a blue light-emitting diode (LED) on P. aeruginosa in vitro. It also explores the synergistic effects of combining TONS504-PACT with EDTA, and compares the antibacterial effects of TONS504-PACT using blue light with those of previously reported red light. Methods To evaluate the antibacterial effects of TONS504-PACT using blue light on P. aeruginosa, PACT with TONS504 (0–100 mg/L) was applied using a 405 nm LED at 0–30 J/cm2 to P. aeruginosa (107–8 CFU/mL). The antibacterial effects were assessed by calculating the survival fraction. Subsequently, to compare the effects of TONS504-PACT using blue light with those of using red light, PACT with TONS504 (10 or 100 mg/L) was conducted using a 405 or 660 nm LED at 30 J/cm2 and evaluated in the same manner. Finally, to investigate the synergistic antibacterial effects of TONS504-PACT with EDTA, PACT with TONS504 (0–100 mg/L) was applied using a 405 nm LED at 5 J/cm2, both with and without EDTA (0.015 M), and its antibacterial effects were similarly assessed. Results TONS504-PACT using blue light decreased the survival of P. aeruginosa, depending on the light intensity. The greatest antibacterial effect was observed at 10 mg/L and 30 J/cm2, showing a survival fraction of 3.5 × 10-5. TONS504-PACT demonstrated a significantly greater antibacterial effect using blue light than that demonstrated using red light at 10 mg/L, though not at 100 mg/L. The combination of TONS504-PACT using blue light with EDTA significantly enhanced antibacterial effects at all concentrations. Conclusion These findings confirm the effectiveness of TONS504-PACT using blue light against P. aeruginosa and suggest its potential as a treatment for bacterial keratitis.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Medical Press","subitem_publisher_language":"en"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Yonago Acta Medica. 2024, 67(4), 284-292."}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam67-4/67-4contents.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam67-4/67-4contents.html","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.33160/yam.2024.11.001"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.33160/yam.2024.11.001","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C)2024 Tottori University Medical 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Norihiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ito","familyNameLang":"en"}],"givenNames":[{"givenName":"Norihiko","givenNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"15101","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"Tottori University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Harada, Kazuki","creatorNameLang":"en"},{"creatorName":"原田, 和記","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Harada","familyNameLang":"en"},{"familyName":"原田","familyNameLang":"ja"}],"givenNames":[{"givenName":"Kazuki","givenNameLang":"en"},{"givenName":"和記","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"80549543","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80549543"},{"nameIdentifier":"100000885","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000885_ja.html"}]},{"creatorNames":[{"creatorName":"Sakata, Isao","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sakata","familyNameLang":"en"}],"givenNames":[{"givenName":"Isao","givenNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"15101","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"Tottori University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Osaki, Tomohiro","creatorNameLang":"en"},{"creatorName":"大崎, 智弘","creatorNameLang":"ja"}],"familyNames":[{"familyName":"Osaki","familyNameLang":"en"},{"familyName":"大崎","familyNameLang":"ja"}],"givenNames":[{"givenName":"Tomohiro","givenNameLang":"en"},{"givenName":"智弘","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"40431332","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40431332"},{"nameIdentifier":"100000602","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000602_ja.html"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-01-17"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2024-11-27"}],"filename":"yam67(4)_284.pdf","filesize":[{"value":"874 KB"}],"format":"application/pdf","licensefree":"(C) 2024 Tottori University Medical Press","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yam67(4)_284.pdf","objectType":"fulltext","url":"https://repository.lib.tottori-u.ac.jp/record/2001684/files/yam67(4)_284.pdf"},"version_id":"ab63d98e-3ca0-4c19-97fd-cc2ec59b16a6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"blue light","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"EDTA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"photochemotherapy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pseudomonas aeruginosa","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TONS504","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In Vitro Efficacy of Photodynamic Antimicrobial Chemotherapy with TONS504 Using Blue Light Against Pseudomonas aeruginosa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In Vitro Efficacy of Photodynamic Antimicrobial Chemotherapy with TONS504 Using Blue Light Against Pseudomonas 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