{"created":"2025-05-28T08:06:36.190018+00:00","id":2001868,"links":{},"metadata":{"_buckets":{"deposit":"e26bb58a-48dc-4756-a6c8-1ed4806f67e0"},"_deposit":{"created_by":85,"id":"2001868","owner":"85","owners":[85],"pid":{"revision_id":0,"type":"depid","value":"2001868"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:02001868","sets":["1:10","2:12"]},"author_link":[],"control_number":"2001868","item_1698624010":{"attribute_name":"出版者情報","attribute_value_mlt":[{"publisher_names":[{"publisher_name":"Elsevier","publisher_name_language":"en"}]}]},"item_30002_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"embargoed access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_f1cf"}]},"item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-05-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5-6","bibliographicPageStart":"184422","bibliographicVolumeNumber":"1867","bibliographic_titles":[{"bibliographic_title":"Biochimica et Biophysica Acta (BBA) - Biomembranes","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"15101","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"鳥取大学","affiliationNameLang":"ja"},{"affiliationName":"Tottori University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Hanashima,Shinya","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"花島,慎弥","creatorNameLang":"ja","creatorNameType":"Personal"}],"nameIdentifiers":[{"nameIdentifier":"100002461_ja.html","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100002461_ja.html"},{"nameIdentifier":"50373353","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50373353"}]},{"creatorNames":[{"creatorName":"Asahina,Takafumi","creatorNameLang":"en","creatorNameType":"Personal"}]},{"creatorNames":[{"creatorName":"Malabed,Raymond","creatorNameLang":"en","creatorNameType":"Personal"}]},{"creatorNames":[{"creatorName":"Sasaki,Katsuaki","creatorNameLang":"en","creatorNameType":"Personal"}]},{"creatorNames":[{"creatorName":"Murata,Michio","creatorNameLang":"en","creatorNameType":"Personal"}],"nameIdentifiers":[{"nameIdentifier":"40183652","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40183652"}]}]},"item_30002_date11":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2025-05-08","subitem_date_issued_type":"Issued"}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Cholesterol (Chol) in mammalian cell membranes facilitates the assembly of dynamic membrane domains that are involved　in vital biological processes through lateral and transbilayer movements in the membranes. In the cell membranes, Chol　undergoes glucose transglycosylation to produce cholesteryl-β-d-glucoside (ChoGlc). ChoGlc is involved in　neurodegenerative diseases and accumulates in lysosomal storage disorders. However, the effects of glucosylation on　membrane properties of Chol remain unclear. We investigated the membrane interaction of ChoGlc and its subsequent　translocation between leaflets using fluorescent probes, such as 4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene　(TMA-DPH) and the newly synthesized 7-nitro-2,1,3-benzoxadiazole-labeled ChoGlc (NBD-ChoGlc) in　dioleoylphosphatidylcholine (DOPC) membranes. The fluorescence of TMA-DPH, which selectively reported the order of　the outer leaflet of the bilayer, indicated that ChoGlc added to the external solution, was mostly incorporated into the　membranes and increased the DOPC membrane ordering. Furthermore, the anisotropy values reached a level similar to　that of the ChoGlc-preloaded symmetric vesicle within approximately 5 min owing to the rapid distribution of ChoGlc in both　leaflets. This was further confirmed by the selective fluorescence quenching of NBD-ChoGlc in the outer leaflet through　irreversible quenching by dithionite. The similarity of the fluorescence decay curves of NBD-ChoGlc and NBD-Chol　indicated that the glucosylation had little impact on the flip-flops of Chol in the DOPC bilayers. Our data demonstrates that　some of the important membrane properties of Chol, such as fast flip-flop between leaflets and increased membrane order,　were mostly maintained in ChoGlc despite hydrophilic glucose modification.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2026-05-07"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2025-05-08"}],"filename":"bbamem1867(5-6)_184422.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://repository.lib.tottori-u.ac.jp/record/2001868/files/bbamem1867(5-6)_184422.pdf"},"version_id":"e1498a70-44a7-4355-b445-7a33baaa7ee1"}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_30002_relation18":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbamem.2025.184422","subitem_relation_type_select":"DOI"}}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30002_rights6":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"Copyright © 2025 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"18792642","subitem_source_identifier_type":"EISSN"}]},"item_30002_subject8":{"attribute_name":"主題","attribute_value_mlt":[{"subitem_subject":"Cholesterol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Steryl glucoside","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glucosylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bilayer membrane","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Flip-flop","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of glucosylation for the vertical movement of cholesterol in bilayer membranes","subitem_title_language":"en"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_title":"Effect of glucosylation for the vertical movement of cholesterol in bilayer membranes","item_type_id":"30002","owner":"85","path":["10","12"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-05-28"},"publish_date":"2025-05-28","publish_status":"0","recid":"2001868","relation_version_is_last":true,"title":["Effect of glucosylation for the vertical movement of cholesterol in bilayer membranes"],"weko_creator_id":"85","weko_shared_id":-1},"updated":"2025-06-03T09:35:16.013217+00:00"}