{"created":"2023-08-02T03:55:40.471270+00:00","id":4604,"links":{},"metadata":{"_buckets":{"deposit":"2acf61b7-95a3-45bc-99e3-0769a769471e"},"_deposit":{"created_by":10,"id":"4604","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"4604"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00004604","sets":["1:9","2:12","23:34:975:976"]},"author_link":["2980","4779","4668","2885"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-02-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"18","bibliographicPageStart":"7","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"Yonago Acta Medica"},{"bibliographic_title":"Yonago Acta Medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Canine mammary gland tumors can be used as predictive models for human breast cancer. There are several types of microRNAs common in human breast cancer and canine mammary gland tumors. The functions of microRNAs in canine mammary gland tumors are not well understood. Methods: We compared the characterization of microRNA expression in two-dimensional and three-dimensional canine mammary gland tumor cell models. We evaluated the differences between two- and three-dimensional cultured canine mammary gland tumor SNP cells by assessing microRNA expression levels, morphology, drug sensitivity, and hypoxia. Results: The expression of microRNA-210 in the three-dimensional-SNP cells was 10.19 times higher than that in the two-dimensional-SNP cells. The intracellular concentrations of doxorubicin in the two- and three-dimensional-SNP cells were 0.330 ± 0.013 and 0.290 ± 0.048 nM/mg protein, respectively. The IC50 values of doxorubicin for the two- and three-dimensional-SNP cells were 5.2 and 1.6 μM, respectively. Fluorescence of the hypoxia probe, LOX-1, was observed inside the sphere of three-dimensional-SNP cells without echinomycin but not in two-dimensional-SNP cells. The three-dimensional-SNP cells treated with echinomycin showed weak LOX-1 fluorescence. Conclusion: The present study showed a clear difference in microRNA expression levels in cells cultured in a two-dimensional adherent versus a three-dimensional spheroid model.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Medical Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.33160/yam.2023.02.002","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta Medica. 2023, 66(1), 7-18. doi:10.33160/yam.2023.02.002"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.33160/yam.2023.02.002"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.33160/yam.2023.02.002","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2023 Tottori University Medical Press."}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05135710","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"13468049"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"尾崎, 知博"},{"creatorName":"オサキ, トモヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Osaki, Tomohiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2980","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10457161","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10457161"}]},{"creatorNames":[{"creatorName":"寸田, 祐嗣"},{"creatorName":"スンデン, ユウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Sunden, Yuji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4779","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20451403","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20451403"},{"nameIdentifier":"100001167","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001167_ja.html"}]},{"creatorNames":[{"creatorName":"割田, 克彦"},{"creatorName":"ワリタ, カツヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Warita, Katsuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4668","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40452669","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40452669"},{"nameIdentifier":"100001516","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001516_ja.html"}]},{"creatorNames":[{"creatorName":"岡本, 芳晴"},{"creatorName":"オカモト, ヨシハル","creatorNameLang":"ja-Kana"},{"creatorName":"Okamoto, Yoshiharu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2885","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50194410","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50194410"},{"nameIdentifier":"100000607","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000607_ja.html"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-15"}],"displaytype":"detail","filename":"yam66(1)_7.pdf","filesize":[{"value":"6.6 MB"}],"format":"application/pdf","licensefree":"(C) 2023 Tottori University Medical Press.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yam66(1)_7.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/4604/files/yam66(1)_7.pdf"},"version_id":"2a34dd94-7547-4103-928d-8bad176a4503"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"canine","subitem_subject_scheme":"Other"},{"subitem_subject":"mammary gland tumor","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"three-dimensional","subitem_subject_scheme":"Other"},{"subitem_subject":"two-dimensional","subitem_subject_scheme":"Other"},{"subitem_subject":"canine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mammary gland tumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"three-dimensional","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"two-dimensional","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparison of Characterization in Two-Dimensional and Three-Dimensional Canine Mammary Gland Tumor Cell Models","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparison of Characterization in Two-Dimensional and Three-Dimensional Canine Mammary Gland Tumor Cell Models","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","976"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-28"},"publish_date":"2023-02-28","publish_status":"0","recid":"4604","relation_version_is_last":true,"title":["Comparison of Characterization in Two-Dimensional and Three-Dimensional Canine Mammary Gland Tumor Cell Models"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-28T23:45:33.043964+00:00"}