{"created":"2023-08-02T03:55:40.605577+00:00","id":4606,"links":{},"metadata":{"_buckets":{"deposit":"90881a9a-8534-4122-9773-dee87865f8d7"},"_deposit":{"created_by":10,"id":"4606","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"4606"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00004606","sets":["1:9","2:12","23:34:975:976"]},"author_link":["1469","743","16485","16486"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-02-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"35","bibliographicPageStart":"24","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"Yonago Acta Medica"},{"bibliographic_title":"Yonago Acta Medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Cytoplasmic mislocalization of TAR-DNA binding protein of 43 kDa (TDP-43) is a major hallmark of amyotrophic lateral sclerosis (ALS). TDP-43 aggregation is detected in the cortical and spinal motor neurons in most ALS cases; however, pathological mechanism of this mislocalized TDP-43 remains unknown. Methods: We generated a tetracycline-inducible TDP-43 A315T system on a mouse artificial chromosome (MAC) vector to avoid transgene-insertional mutagenesis, established a mouse embryonic stem (ES) cell line holding this MAC vector system, and investigated whether overexpressed exogenous TDP-43 A315T was mislocalized in the cytoplasm of the ES cell-derived neurons and triggered the neurotoxic effects on these cells. Results: Inducible TDP-43 A315T system was successfully loaded onto the MAC and introduced into the mouse ES cells. These ES cells could differentiate into motor neurons and interneurons. Overexpression of TDP-43 A315T by addition of doxycycline in both neurons resulted in mislocalization to cytoplasm. Mislocalized TDP-43 caused cell death of motor neurons, but not interneurons. Conclusion: Vulnerability to cytoplasmic mislocalized TDP-43 is selective on neuronal types, whereas mislocalization of overexpressed TDP-43 occurs in even insusceptible neurons. This inducible gene expression system using MAC remains useful for providing critical insights into appearance of TDP-43 pathology.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Medical Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.33160/yam.2023.02.004","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta Medica. 2023, 66(1), 24-35. doi:10.33160/yam.2023.02.004"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.33160/yam.2023.02.004"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.33160/yam.2023.02.004","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2023 Tottori University Medical Press."}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05135710","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"13468049"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Chromosome Engineering Research Center, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University / Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University / Chromosome Engineering Research Group, The Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences"},{"subitem_text_language":"en","subitem_text_value":"Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"香月, 康宏"},{"creatorName":"カズキ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Kazuki, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"平塚, 正治"},{"creatorName":"ヒラツカ, マサハル","creatorNameLang":"ja-Kana"},{"creatorName":"Hiratsuka, Masaharu","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Togai, Shota","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hamamichi, Shusei","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-15"}],"displaytype":"detail","filename":"yam66(1)_24.pdf","filesize":[{"value":"6.2 MB"}],"format":"application/pdf","licensefree":"(C) 2023 Tottori University Medical Press.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yam66(1)_24.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/4606/files/yam66(1)_24.pdf"},"version_id":"14cb2aaa-c728-4361-958b-4e924f5d9705"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"doxycycline","subitem_subject_scheme":"Other"},{"subitem_subject":"interneuron","subitem_subject_scheme":"Other"},{"subitem_subject":"motor neuron","subitem_subject_scheme":"Other"},{"subitem_subject":"mouse artificial chromosome","subitem_subject_scheme":"Other"},{"subitem_subject":"TAR-DNA binding protein of 43 kDa","subitem_subject_scheme":"Other"},{"subitem_subject":"doxycycline","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"interneuron","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"motor neuron","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mouse artificial chromosome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TAR-DNA binding protein of 43 kDa","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","976"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-02-28"},"publish_date":"2023-02-28","publish_status":"0","recid":"4606","relation_version_is_last":true,"title":["Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-28T23:45:40.566789+00:00"}