{"created":"2023-08-02T03:55:41.861560+00:00","id":4629,"links":{},"metadata":{"_buckets":{"deposit":"090d0c65-2594-4275-8903-4b4499cb950b"},"_deposit":{"created_by":10,"id":"4629","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"4629"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00004629","sets":["1:9","2:12","23:34:978:979"]},"author_link":["3099","4074","16563"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"52","bibliographicPageStart":"44","bibliographicVolumeNumber":"65","bibliographic_titles":[{"bibliographic_title":"Yonago Acta Medica"},{"bibliographic_title":"Yonago Acta Medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Mammary serine protease inhibitor (maspin) is well known as a tumor suppressor gene in several types of cancers and its nuclear localization is essential for its tumor-suppressive function. We previously reported that the cytoplasmic-only localization of maspin is significantly correlated with unfavorable prognosis in patients with lung adenocarcinoma (LUAD). To clarify whether maspin in LUAD acts as a tumor promoter or suppressor, we examined the subcellular localization-dependent biological functions of maspin in human LUAD cell lines. Methods: The expression levels and subcellular localization of maspin were investigated by performing immunoblotting and immunofluorescence in human LUAD cell lines (PC-9, A549, NCI-H23, RERF-LCKJ) and human bronchial epithelial cell line (BEAS-2B). We then established stable cell lines overexpressing maspin (A549-maspin and RERF-LC-KJ-maspin) and investigated their subcellular localization. Cell invasion assays of these cell lines were performed to examine their invasiveness. Moreover, the mRNA expression levels between epithelial cell markers (E-cadherin) and mesenchymal cell markers (N-cadherin and vimentin) were compared. Results: The expression of maspin in PC-9 cells was comparable to that in BEAS-2B cells, whereas its expression in A549, NCI-H23, and RERF-LC-KJ cells was decreased. The cell invasion capability of A549-maspin cells showing pancellular expression was significantly decreased compared with that of A549-control cells. By contrast, the cell invasion capability of RERF-LC-KJmaspin cells showing cytoplasmic-only expression was significantly increased compared with that of RERFLC-KJ-control cells. The mRNA expression levels of N-cadherin, but not E-cadherin and vimentin, in A549-maspin cells was significantly downregulated compared with that in A549-control cells. No significant differences in these markers were observed between RERFLC-KJ-maspin and RERF-LC-KJ-control cells. Conclusion: The invasive capability of LUAD cells is regulated by the intracellular localization of maspin. Clarification of the molecular mechanism underlying the subcellular localization-dependent function of maspin will promote a deeper understanding of LUAD development and progression.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Medical Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.33160/yam.2022.02.006","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta Medica. 2022, 65(1), 44-52. doi:10.33160/yam.2022.02.006"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam65-1/65-1contents.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.lib.tottori-u.ac.jp/yam/yam/yam65-1/65-1contents.html","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.33160/yam.2022.02.006"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.33160/yam.2022.02.006","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2022 Tottori University Medical Press."}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05135710","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"13468049"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"坂部, 友彦"},{"creatorName":"サカベ, トモヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakabe, Tomohiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3099","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50639747","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50639747"},{"nameIdentifier":"100001095","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001095_ja.html"}]},{"creatorNames":[{"creatorName":"梅北, 善久"},{"creatorName":"ウメキタ, ヨシヒサ","creatorNameLang":"ja-Kana"},{"creatorName":"Umekita, Yoshihisa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4074","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80244226","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80244226"},{"nameIdentifier":"100000852","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000852_ja.html"}]},{"creatorNames":[{"creatorName":"Matsushige, Takahiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16563","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-15"}],"displaytype":"detail","filename":"yam65(1)_44.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensefree":"(C) 2022 Tottori University Medical Press.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yam65(1)_44.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/4629/files/yam65(1)_44.pdf"},"version_id":"f532337c-a6ff-46df-9235-4ddeb525c992"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"lung adenocarcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"maspin","subitem_subject_scheme":"Other"},{"subitem_subject":"lung adenocarcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"maspin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","979"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-03-09"},"publish_date":"2022-03-09","publish_status":"0","recid":"4629","relation_version_is_last":true,"title":["Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-28T23:46:56.680361+00:00"}