{"created":"2023-08-02T03:55:46.561878+00:00","id":4721,"links":{},"metadata":{"_buckets":{"deposit":"3b21a6c1-8e2d-49d7-91cf-8916412d56a4"},"_deposit":{"created_by":10,"id":"4721","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"4721"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00004721","sets":["1:9","2:12","23:34:988:989"]},"author_link":["16863","16864","1087","2273","1082","3368"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-02-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"87","bibliographicPageStart":"79","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"Yonago Acta Medica"},{"bibliographic_title":"Yonago Acta Medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Liver fibrosis progresses to decompensated liver cirrhosis, for which medical needs remain unmet. We recently developed IC-2, a small-molecule compound that suppresses Wnt/β-catenin signaling, and found that IC-2 also suppresses liver fibrosis. In this study, we performed three-step screening of newly synthesized IC-2 derivatives to identify other small-molecule compounds that suppress liver fibrosis. Methods: The screening system consisted of three steps: a cell viability assay, a transcription factor 4 (TCF4) reporter assay, and induction of α-smooth muscle actin (α-SMA) and collagen 1α1 (Col1A1) expression in response to each compound. Screening using human LX-2 hepatic stellate cells (HSCs) was performed to target HSCs, which are the driver cells of liver fibrosis. Results: In the first step, since 9b and 9b-CONH2 at 100 μM did not have any effects on cell viability, they were omitted in the next screening. Additionally, the conditions that led to > 40% inhibition of the controls were also excluded in subsequent screening. The second step was performed under 31 conditions for 19 small-molecule compounds. Sixteen small-molecule compounds caused significant reduction of TCF4 activity relative to that of 0.1% DMSO. Of the 16 compounds, the 10 showing the greatest suppression of TCF4 activity were selected for the third step. Expressions of mRNA for α-SMA and Col1A1 were significantly reduced by seven and three small-molecule compounds, respectively. The greatest reductions in the α-SMA and Col1A1 mRNA expressions were observed in the cells treated with IC-2-F. Protein expressions of α-SMA and Col1A1 caused by IC-2-F were also comparable to those caused by IC-2. Conclusion: IC-2-F was identified as a novel deactivating small-molecule compound for HSCs in vitro. These data suggest that IC-2-F is a promising medicine for liver fibrosis.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Medical Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.33160/yam.2020.02.013","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Takuki Sakaguchi, Yohei Kono, Noriko Itaba, Minoru Morimoto, Hajime Isomoto, Goshi Shiota, Identification of a Novel Deactivating Small-Molecule Compound for Fibrogenic Hepatic Stellate Cells, Yonago Acta Medica, 2020, Volume 63, Issue 1, Pages 79-87, Rel"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.jstage.jst.go.jp/article/yam/63/1/63_2020.02.013/_article/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/yam/63/1/63_2020.02.013/_article/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.lib.tottori-u.ac.jp/yam/yam/yam63-1/63-1contents.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.lib.tottori-u.ac.jp/yam/yam/yam63-1/63-1contents.html","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05135710","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"1346-8049"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medical Sciences, Tottori University / Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medical Sciences, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medical Sciences, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Research Initiative Center, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medical Sciences, Tottori University"}]},"item_10001_text_34":{"attribute_name":"雑誌内区分","attribute_value_mlt":[{"subitem_text_value":"Original Article"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakaguchi, Takuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kono, Yohei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Itaba, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Morimoto, Minoru","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Isomoto, Hajime","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Shiota, Goshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-15"}],"displaytype":"detail","filename":"yam63(1)_79.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yam63(1)_79.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/4721/files/yam63(1)_79.pdf"},"version_id":"91200415-ea39-4753-b44a-af1342591e7b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"deactivation","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatic stellate cells","subitem_subject_scheme":"Other"},{"subitem_subject":"liver fibrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"small-molecule compound","subitem_subject_scheme":"Other"},{"subitem_subject":"deactivation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatic stellate cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liver fibrosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"small-molecule compound","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Identification of a Novel Deactivating Small-Molecule Compound for Fibrogenic Hepatic Stellate Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of a Novel Deactivating Small-Molecule Compound for Fibrogenic Hepatic Stellate Cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","989"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-02-28"},"publish_date":"2020-02-28","publish_status":"0","recid":"4721","relation_version_is_last":true,"title":["Identification of a Novel Deactivating Small-Molecule Compound for Fibrogenic Hepatic Stellate Cells"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-28T23:52:03.669602+00:00"}