{"created":"2023-08-02T03:55:49.716086+00:00","id":4773,"links":{},"metadata":{"_buckets":{"deposit":"b4359980-9aa4-4450-b0a6-d79ae710eb57"},"_deposit":{"created_by":10,"id":"4773","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"4773"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00004773","sets":["1:9","2:12","23:34:993:994"]},"author_link":["17106","17107","17108","17109","3616","17110","17111","4846","4358","17112","17113","17114","17115","17116","17117"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"93","bibliographicPageStart":"85","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"Yonago Acta Medica"},{"bibliographic_title":"Yonago Acta Medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[Background] The epidermal growth factor receptor (EGFR) is a therapeutic target for patients with non-small cell lung cancer (NSCLC). Cetuximab is an anti-EGFR monoclonal antibody that inhibits EGFR signaling and proliferation of colorectal cancer and head and neck cancers. Since only few NSCLC patients benefit from cetuximab therapy, we evaluated a novel combination treatment using cetuximab and EGFR small interfering RNA (siRNA) to strongly suppress EGFR signaling and searched for a biomarker in NSCLC cell lines harboring wild-type EGFR. [Methods] Alterations in EGFR and its downstream genes in five NSCLC cell lines (A549, Lu99, 86-2, Sq19 and Ma10) were assessed through sequencing. The protein expression levels of these molecules were assessed through western blotting. The effect of combination treatment was determined through cell proliferation assay, caspase-3/7 assay, invasion assay, and migration assay. [Results] All cell lines were harboring wild-type EGFR, whereas KRAS, PTEN, TP53 and TP53 were mutated in A549 and Lu99; Lu99 and Sq19; Lu99, 86-2, Sq19 and Ma10; and A549, 86-2, and Sq19 cell lines, respectively. PTEN was not expressed in Sq19, and LKB1 was not expressed in both A549 and Sq19. TP53 was not expressed in both A549 and Lu99. The combination of cetuximab and EGFR siRNA significantly suppressed cell proliferation in 86-2, Sq19 and Ma10, which express wild-type KRAS. It induced apoptosis in A549, 86-2 and Ma10 cells, which express wild type PTEN. The combination treatment had no effect either on cell invasion nor migration in all cell lines. [Conclusion] EGFR targeted therapy using the combination of cetuximab and EGFR siRNA is effective in NSCLC cell lines harboring wild-type EGFR. Wild-type KRAS may act as a potential biomarker for response to combination treatment by the induction of apoptosis in cells with wild-type PTEN.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Medical Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.33160/yam.2019.03.012","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta Medica. 2019, 62(1), 85-93"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.jstage.jst.go.jp/article/yam/62/1/62_2019.03.012/_article/-char/en"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/yam/62/1/62_2019.03.012/_article/-char/en","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.lib.tottori-u.ac.jp/yam/yam/yam62-1/62-1contents.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.lib.tottori-u.ac.jp/yam/yam/yam62-1/62-1contents.html","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05135710","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"1346-8049"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"},{"subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine"},{"subitem_text_value":"Department of Pathobiological Science and Technology, School of Health Science, Tottori University Faculty of Medicine"},{"subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"},{"subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_language":"en","subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Department of Pathobiological Science and Technology, School of Health Science, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_language":"en","subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_language":"en","subitem_text_value":"Division of Infectious Diseases, Tottori University Hospital"},{"subitem_text_language":"en","subitem_text_value":"Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miyake, Naomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chikumi, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Kosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takata, Miyako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takata, Miki"},{"creatorName":"タカタ, ミキ","creatorNameLang":"ja-Kana"},{"creatorName":"Takata, Miki","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Okada, Kensaku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitaura, Tsuyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamoto, Masaki"},{"creatorName":"ナカモト, マサキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamoto, Masaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Yamasaki, Akira"},{"creatorName":"ヤマサキ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamasaki, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Miyake, Naomi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chikumi, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Kosuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takata, Miyako","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okada, Kensaku","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitaura, Tsuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-05-22"}],"displaytype":"detail","filename":"yam62(1)_85.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yam62(1)_85.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/4773/files/yam62(1)_85.pdf"},"version_id":"d24de5fb-74be-4194-a570-8ba9c202a65c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cetuximab","subitem_subject_scheme":"Other"},{"subitem_subject":"EGFR siRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"KRAS","subitem_subject_scheme":"Other"},{"subitem_subject":"nonsmall cell lung cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"cetuximab","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"EGFR siRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"KRAS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nonsmall cell lung cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Effect of Cetuximab and EGFR Small Interfering RNA Combination Treatment in NSCLC Cell Lines with Wild Type EGFR and Use of KRAS as a Possible Biomarker for Treatment Responsiveness","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of Cetuximab and EGFR Small Interfering RNA Combination Treatment in NSCLC Cell Lines with Wild Type EGFR and Use of KRAS as a Possible Biomarker for Treatment Responsiveness","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","994"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-06-17"},"publish_date":"2019-06-17","publish_status":"0","recid":"4773","relation_version_is_last":true,"title":["Effect of Cetuximab and EGFR Small Interfering RNA Combination Treatment in NSCLC Cell Lines with Wild Type EGFR and Use of KRAS as a Possible Biomarker for Treatment Responsiveness"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-28T23:55:03.807856+00:00"}