{"created":"2023-08-02T03:56:06.036297+00:00","id":5082,"links":{},"metadata":{"_buckets":{"deposit":"03c1c1b1-8059-4ad2-b6d1-58d96b4a44dc"},"_deposit":{"created_by":10,"id":"5082","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5082"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005082","sets":["1:9","2:12","23:34:1063:1066"]},"author_link":["18788","2976","3066","18789"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"74","bibliographicPageStart":"63","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"Yonago Acta medica"},{"bibliographic_title":"Yonago Acta medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The tumoricidal effect of herpes simplex virus thymidine kinase (HSV-tk) gene transfer followed by ganciclovir (GCV) treatment has been demonstrated in relation to the bystander effect. We examined the mode of cell death after GCV treatment in three human oral squamous cell carcinoma (SCC) cell lines that had been infected with adenovirus possessing HSV-tk gene. Oral SCC cell lines displayed high susceptibility to HSV-tk/GCV treatment despite low transduction. Evidence suggests that apoptosis was not found by internucleosomal DNA fragmentation, cell cycle kinetics or gene expressions indicative of apoptosis. However, we observed different levels of expression of annexin V-positive/propidium iodide-negative cells in all the cell lines and occasional TUNEL-positive cells in one cell line. To address these controversial findings, we further confirmed the morphological phenotypes of cell death with semi-thin and ultra-thin sections, which revealed that the cells undergoing death were consistent with necrosis, i.e. swelling of cytoplasm and intracellular organs with membrane disruption. Furthermore, no apoptotic bodies were detected within cytoplasm of apparently intact cells. A nonapoptotic mechanism may play a central role in the HSV-tk/GCV-induced cell death in oral SCC cell lines.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Faculty of Medicine"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta medica. 2005, 48(3), 63-74"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468049","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Division of Organ Pathology, Department of Microbiology and Pathology,Tottori University Faculty of Medicine. Division of Oral and Maxillofacial Biopathological Surgery, Department of Medicine of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine"},{"subitem_text_value":"Division of Organ Pathology, Department of Microbiology and Pathology,Tottori University Faculty of Medicine"},{"subitem_text_value":"Division of Oral and Maxillofacial Biopathological Surgery, Department of Medicine of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kuratate, Itaru"}],"nameIdentifiers":[{"nameIdentifier":"18788","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Osaki, Mitsuhiko"},{"creatorName":"Osaki, Mitsuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2976","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40325006","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40325006"},{"nameIdentifier":"100000119","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000119_ja.html"}]},{"creatorNames":[{"creatorName":"Ryoke, Kazuo"},{"creatorName":"Ryoke, Kazuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3066","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20093635","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20093635"}]},{"creatorNames":[{"creatorName":"Kuratate, Itaru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18789","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-06-09"}],"displaytype":"detail","filename":"48_063-074.pdf","filesize":[{"value":"326.7 kB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"48_063-074.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/5082/files/48_063-074.pdf"},"version_id":"e17a0db2-1f07-4b0f-b595-a919cd6877d5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"ganciclovir","subitem_subject_scheme":"Other"},{"subitem_subject":"necrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"thymidine kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ganciclovir","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"necrosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"thymidine kinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cell Death Induction of Thymidine Kinase Gene Transfer Followed by Ganciclovir Treatment in Oral Squamous Cell Carcinoma Cell Lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cell Death Induction of Thymidine Kinase Gene Transfer Followed by Ganciclovir Treatment in Oral Squamous Cell Carcinoma Cell Lines","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","1066"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5082","relation_version_is_last":true,"title":["Cell Death Induction of Thymidine Kinase Gene Transfer Followed by Ganciclovir Treatment in Oral Squamous Cell Carcinoma Cell Lines"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-10-03T23:53:11.487461+00:00"}