{"created":"2023-08-02T03:56:07.945266+00:00","id":5112,"links":{},"metadata":{"_buckets":{"deposit":"0790a495-5142-4847-8998-02005aec1691"},"_deposit":{"created_by":10,"id":"5112","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5112"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005112","sets":["1:9","2:12","23:34:1073:1077"]},"author_link":["18956","18957"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"115","bibliographicPageStart":"109","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Yonago Acta medica"},{"bibliographic_title":"Yonago Acta medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Following estrogen replacement in old female rats, changes in apoptosis-related genes (Bcl-2, Bax) in the urethra were investigated by immunohistochemical and RT-PCR analysis. A total of 40 old (16-month-old) female Wistar rats were divided into the following 5 groups: normal controls; rats replaced with low-dose estradiol (E2) for 2 and 4 weeks, respectively; and rats replaced with high-dose E2 for 2 and 4 weeks, respectively. After treatment, the urethras were removed, weighed and stained with hematoxylin and eosin, and then immunohistochemical stainings of Bcl-2 and Bax were performed. In addition,the expressions of the apoptosis-related genes bcl-2 and bax were investigated by PCR analysis, and then evaluated by computerized quantification. In the E2-replaced rats, the immunostained urethras showed little immunoreactivity against the Bcl-protein, and inthe control rats, no reactivity was seen. In contrast, the control rats showedimmunoreactivity against the Bax protein, whereas the E2-replaced rats showed little reactivity. Upon mRNA analysis, the expression of bcl-2 mRNA was uprisen in rats in the high-dose E2 for 4 weeks group as compared with the controls, but the expression of bax mRNA was suppressed in the E2-replaced rats as compared with the controls. As these results suggest, estrogen replacement up-regulated the expression of bcl-2 mRNA and down-regulated the expression of bax mRNA, which might suppress the apoptic action: these changes might alter lower urinary tract findings.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Faculty of Medicine"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta medica. 2003, 46(4), 109-115"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468049","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kinouchi, Shinichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kinouchi, Shinichiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-06-09"}],"displaytype":"detail","filename":"46_109-115.pdf","filesize":[{"value":"134.9 kB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"46_109-115.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/5112/files/46_109-115.pdf"},"version_id":"2d1e5ac8-b555-4496-bc69-72ae10cce0b2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"estrogen replacement","subitem_subject_scheme":"Other"},{"subitem_subject":"old rat","subitem_subject_scheme":"Other"},{"subitem_subject":"urethra","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"estrogen replacement","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"old rat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"urethra","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Changes in Apoptosis-Related Genes (Bcl-2, Bax) in the Urethras of Old Female Rats Following Estrogen Replacement","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Changes in Apoptosis-Related Genes (Bcl-2, Bax) in the Urethras of Old Female Rats Following Estrogen Replacement","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","1077"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5112","relation_version_is_last":true,"title":["Changes in Apoptosis-Related Genes (Bcl-2, Bax) in the Urethras of Old Female Rats Following Estrogen Replacement"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-10-03T23:54:48.556077+00:00"}