{"created":"2023-08-02T03:56:10.997753+00:00","id":5167,"links":{},"metadata":{"_buckets":{"deposit":"8084a907-6fdf-402e-8ab6-46fd12838222"},"_deposit":{"created_by":10,"id":"5167","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5167"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005167","sets":["1:9","2:12","23:34:1091:1092"]},"author_link":["19200","19201","19202","19203","19204","19205"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"20","bibliographicPageStart":"11","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Yonago Acta medica"},{"bibliographic_title":"Yonago Acta medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to clarify the effects of dexamethasone on epithelial cells at the transcription factor level, protein synthesis level and functional level, we examined neutrophil adhesion to A549 cells (an immortalized human type II alveolar epithelial cell line), expression of intercellular adhesion molecule-1 (ICAM-1), and translocation of nuclear factor-kappa B (NF-κB) into nuclei. The intranuclear localization of fluorescently stained NF-κB and an adhesion assay between neutrophils and A549 cells was analyzed quantitatively using laser scanning cytometry. ICAM-1 expression on A549 cells was measured by flow cytometric analysis. Stimulation of A549 cells with tumor necrosis factor (TNF)-α caused a time- and dose-dependent increase in their adhesiveness for neutrophils. ICAM-1 expression on A549 cells after stimulation with TNF-α for 12 h was significantly up-regulated, compared with unstimulated cells (P< 0.05). Intranuclear NF-κB was induced 10 min after stimulation with TNF-α and was increased in a TNF-α-dose-dependent manner. Dexamethasone suppressed TNF-α-induced NF-κB translocation in A549 cells by approximately 60%, ICAM-1 expression on A549 cells by approximately 40%, and A549 adhesiveness for neutrophils completely. Therefore, we suggest that the inhibition of A549 adhesiveness for neutrophils by dexamethasone may be due in part to the suppression of NF-κB entry into nuclei and ICAM-1 expression in A549 cells.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Faculty of Medicine"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta medica. 1999, 42(1), 11-20"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468049","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Third Department of Internal Medicine, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Third Department of Internal Medicine, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Third Department of Internal Medicine, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tokuyasu, Hirokazu"}],"nameIdentifiers":[{"nameIdentifier":"19200","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomita, Katsuyuki"}],"nameIdentifiers":[{"nameIdentifier":"19201","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hitsuda, Yutaka"}],"nameIdentifiers":[{"nameIdentifier":"19202","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tokuyasu, Hirokazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19203","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomita, Katsuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19204","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hitsuda, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19205","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-06-05"}],"displaytype":"detail","filename":"42_011-020.pdf","filesize":[{"value":"220.2 kB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"42_011-020.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/5167/files/42_011-020.pdf"},"version_id":"19501912-a6fe-4247-a809-2ff1e35e6253"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"alveolar epithelial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"adhesion assay","subitem_subject_scheme":"Other"},{"subitem_subject":"ICAM-1","subitem_subject_scheme":"Other"},{"subitem_subject":"laser scanning cytometry","subitem_subject_scheme":"Other"},{"subitem_subject":"neutrophils","subitem_subject_scheme":"Other"},{"subitem_subject":"NF-κB","subitem_subject_scheme":"Other"},{"subitem_subject":"alveolar epithelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"adhesion assay","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ICAM-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"laser scanning cytometry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"neutrophils","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NF-κB","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Inhibitory Effect of Dexamethasone on A549 Cell Adhesion to Neutrophils: Possible Involvement of Nuclear Factor-Kappa B","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Inhibitory Effect of Dexamethasone on A549 Cell Adhesion to Neutrophils: Possible Involvement of Nuclear Factor-Kappa B","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","1092"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5167","relation_version_is_last":true,"title":["An Inhibitory Effect of Dexamethasone on A549 Cell Adhesion to Neutrophils: Possible Involvement of Nuclear Factor-Kappa B"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-10-03T23:57:52.029775+00:00"}