{"created":"2023-08-02T03:56:11.090482+00:00","id":5169,"links":{},"metadata":{"_buckets":{"deposit":"4aaf9d38-adf2-42ff-bc27-7e2d4cb58b0c"},"_deposit":{"created_by":10,"id":"5169","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5169"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005169","sets":["1:9","2:12","23:34:1091:1092"]},"author_link":["19212","19213","19214","2779","19215","19216","19217"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"39","bibliographicPageStart":"31","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Yonago Acta medica"},{"bibliographic_title":"Yonago Acta medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The aims of this study were i) to test the effects of protein kinase C (PKC) activation on the intracellular Ca2+ concentration of rat cardiomyocytes during chemical hypoxia-reoxygenation, ii) to determine the contribution of the sarcoplasmic reticulum (SR) and the Na+-Ca2+ exchange on the regulation of intracellular Ca2+ following PKC activation and iii) to test the role of PKC-dependent intracellular pH changes in intracellular Ca2+ regulation. We used the isolated adult rat cardiomyocyte perfusion model. Cardiomyocytes were loaded with the Ca2+-fluorescent probe Fluo-3 and the pH-fluorescent probe SNARF1. Cells were subjected to 50 min of glucose-free and NaCN chemical hypoxia followed by 30 min of simulated reoxygenation. The activation of PKC significantly inhibited the hypoxia-induced increase of Fluo-3 fluorescent intensity (control; 692 ± 100% and activated PKC; 322 ± 43%: P < 0.05). This inhibitory effect was not affected by the inhibition of the SR Ca2+ uptake induced by thapsigargin, but was cancelled by the inhibition of the Na+-Ca2+ exchange with dichlorobenzamil (thapsigargin 337 ± 47%; dichlorobenzamil 609 ± 100%). PKC activation also attenuated the decrease in intracellular pH during chemical hypoxia, even in the presence of the Na+-H+ exchange inhibitor amiloride (control; 6.54 ± 0.02, PKC; 6.72 ± 0.03, PKC + amiloride; 6.73 ± 0.03). We concluded that the PKC attenuation of Ca2+ overloading to rat cardiomyocytes during chemical hypoxia-reoxygenation does not depend on the Ca2+ uptake by the SR, but does require a Na+-Ca2+ exchange. Since PKC attenuated the increasing intracellular H+ during chemical hypoxia, a low H+ concentration may be important for the maintenance of Ca2+ extrusion via the Na+-Ca2+ exchange.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Faculty of Medicine"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta medica. 1999, 42(1), 31-39"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468049","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Second Department of Surgery, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Second Department of Surgery, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Second Department of Surgery, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Second Department of Surgery, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miyasaka, Shigeto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichiba, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohgi, Shigetsugu"},{"creatorName":"Ohgi, Shigetsugu","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Miyasaka, Shigeto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichiba, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-06-05"}],"displaytype":"detail","filename":"42_031-039.pdf","filesize":[{"value":"82.8 kB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"42_031-039.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/5169/files/42_031-039.pdf"},"version_id":"ca638724-351a-46e2-9238-f96ae7ca263d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ca2+ overload","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular pH","subitem_subject_scheme":"Other"},{"subitem_subject":"isolated rat cardiomyocytes","subitem_subject_scheme":"Other"},{"subitem_subject":"Na+-Ca2+ exchanger","subitem_subject_scheme":"Other"},{"subitem_subject":"protein kinase C","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca2+ overload","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular pH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"isolated rat cardiomyocytes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Na+-Ca2+ exchanger","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein kinase C","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Activated Protein Kinase C Attenuates Ca2+ Overloading and Reoxygenation Hypercontracture in Isolated Rat Cardiomyocytes following Chemical Hypoxia","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Activated Protein Kinase C Attenuates Ca2+ Overloading and Reoxygenation Hypercontracture in Isolated Rat Cardiomyocytes following Chemical Hypoxia","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","1092"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5169","relation_version_is_last":true,"title":["Activated Protein Kinase C Attenuates Ca2+ Overloading and Reoxygenation Hypercontracture in Isolated Rat Cardiomyocytes following Chemical Hypoxia"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-10-03T23:57:58.669006+00:00"}