{"created":"2023-08-02T03:56:11.182453+00:00","id":5171,"links":{},"metadata":{"_buckets":{"deposit":"e90c4e61-ebbe-49a6-bef3-31aaa3dae83b"},"_deposit":{"created_by":10,"id":"5171","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5171"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005171","sets":["1:9","2:12","23:34:1091:1092"]},"author_link":["19222","19223","19224","19225","1414","19226","19227","19228","19229"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"59","bibliographicPageStart":"51","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Yonago Acta medica"},{"bibliographic_title":"Yonago Acta medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Effects of extracellular acidification on intracellular pH (pHi) and ATP-induced calcium mobilization were investigated in rabbit lens epithelial cells. Primary-cultured lens epithelial cells of Japanese white rabbits were used. Intracellular calcium ([Ca2+]i) and pHi were measured by using fluorescent dyes, fura-2 acetoxymethylester (fura-2 AM) and 2',7'-bis (carboxyethyl)-5,6-carboxyfluorescein acetoxymethylester (BCECF AM), respectively. The addition of 10 ?mol/L ATP produced an initial peak followed by a sustained increase in [Ca2+]i in a standard artificial aqueous humor at extracellular pH (pH0) 7.40. The initial peak was abolished by pretreatment with 1 ?mol/L thapsigargin, whereas the sustained increase was attenuated in a Ca2+-free solution or by pretreatment with 100 ?mol/L verapamil. Acidification of the pH0 from 7.40 to 6.80 decreased the pHi from 7.21 to 7.03, and enhanced both the initial peak and sustained increase in [Ca2+]i. These results suggest that acidification of pH0 significantly affects the pHi and modifies the ATP-induced [Ca2+]i transient in rabbit lens epithelial cells.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Faculty of Medicine"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta medica. 1999, 42(1), 51-59"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468049","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Second Department of Physiology and Department of Ophthalmology, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Second Department of Physiology and Department of Ophthalmology, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Department of Ophthalmology, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Second Department of Physiology, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Second Department of Physiology, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Narita, Akiko"}],"nameIdentifiers":[{"nameIdentifier":"19222","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yawata, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"19223","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagata, Masao"}],"nameIdentifiers":[{"nameIdentifier":"19224","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoyama, Yasuaki"}],"nameIdentifiers":[{"nameIdentifier":"19225","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawai, Yasuaki"},{"creatorName":"Kawai, Yasuaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1414","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70143972","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70143972"}]},{"creatorNames":[{"creatorName":"Narita, Akiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19226","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yawata, Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19227","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagata, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19228","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoyama, Yasuaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19229","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-06-08"}],"displaytype":"detail","filename":"42_051-059.pdf","filesize":[{"value":"174.9 kB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"42_051-059.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/5171/files/42_051-059.pdf"},"version_id":"15406173-d90b-4669-8b92-22c6f8ec2b7a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ATP-induced calcium mobilization","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular acidification","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular pH","subitem_subject_scheme":"Other"},{"subitem_subject":"rabbit lens epithelial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP-induced calcium mobilization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular acidification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular pH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rabbit lens epithelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of Extracellular Acidification on Intracellular pH and ATP-Induced Calcium Mobilization in Rabbit Lens Epithelial Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Extracellular Acidification on Intracellular pH and ATP-Induced Calcium Mobilization in Rabbit Lens Epithelial Cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","1092"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5171","relation_version_is_last":true,"title":["Effects of Extracellular Acidification on Intracellular pH and ATP-Induced Calcium Mobilization in Rabbit Lens Epithelial Cells"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-10-03T23:58:05.503443+00:00"}