{"created":"2023-08-02T03:56:11.490687+00:00","id":5178,"links":{},"metadata":{"_buckets":{"deposit":"33ea54d7-be56-4b3c-94c1-b5e641a17048"},"_deposit":{"created_by":10,"id":"5178","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5178"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005178","sets":["1:9","2:12","23:34:1091:1092"]},"author_link":["1319"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"124","bibliographicPageStart":"113","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Yonago Acta medica"},{"bibliographic_title":"Yonago Acta medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ethanol triggers apoptosis in a variety of mammalian cultured cells. Here, ethanol-induced apoptosis in five human gastric carcinoma cell lines was examined. Trypan blue-positive cells were detected in more than 77% of 10% ethanol treated cells at 6 h in all the cell lines. The apoptosis, which had a typical morphology and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) signals were induced with 3% and 5% ethanol treatments. The apoptotic cells were detected in 36% of MKN-74, 54% of MKN-45 which have wild-type p53, and 37% of TMK-1 cells at 48 h with 5% ethanol, in contrast to less than 18% of MKN-28 and KATO-III cells. The DNA ladder appeared in the MKN-74, MKN-45 and TMK-1, but not in the MKN-28 and KATO-III. The expression of apoptosis-associated molecules p53, Bcl-2, Bax and c-Myc proteins was analyzed in the ethanol-treated cells. The levels of p53, Bcl-2 and Bax proteins were not changed in the ethanol-induced apoptosis. MKN-28 and KATO-III showed higher expressions of Bcl-2 protein compared to the other cell lines, and the down-regulation of c-Myc protein after 1.5 h of ethanol treatment. Calcium antagonists verapamil or nifedipine decreased apoptotic cells in ethanol treated MKN-74, MKN-45 and TMK-1. These results suggest: i) 3% and 5% ethanol induced apoptosis via the influx of extracellular calcium, but were not changed the expression levels of p53 and c-Myc protein, while 10% ethanol provoked necrosis in these cell lines, and ii) the apoptosis resistance by ethanol might be implicated in the higher expression of Bcl-2 protein and down-regulation of c-Myc protein as shown in MKN-28 and KATO-III.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Faculty of Medicine"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta medica. 1999, 42(1), 113-124"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468049","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"First Department of Pathology, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kasagi, Noriko"},{"creatorName":"Kasagi, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1319","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60185741","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60185741"},{"nameIdentifier":"100000123","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000123_ja.html"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-06-08"}],"displaytype":"detail","filename":"42_113-124.pdf","filesize":[{"value":"109.5 kB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"42_113-124.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/5178/files/42_113-124.pdf"},"version_id":"350d7349-5d70-4bcd-b00b-e353fdaee6e5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"gastric carcinoma cell line","subitem_subject_scheme":"Other"},{"subitem_subject":"ethanol","subitem_subject_scheme":"Other"},{"subitem_subject":"calcium","subitem_subject_scheme":"Other"},{"subitem_subject":"c-Myc protein","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gastric carcinoma cell line","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ethanol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"calcium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"c-Myc protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","1092"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5178","relation_version_is_last":true,"title":["Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-10-03T23:58:27.039476+00:00"}