{"created":"2023-08-02T03:56:12.583652+00:00","id":5197,"links":{},"metadata":{"_buckets":{"deposit":"1b77714e-ffe2-4487-92cc-634181f814f3"},"_deposit":{"created_by":10,"id":"5197","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5197"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005197","sets":["1:9","2:12","23:34:1095:1096"]},"author_link":["4358","19324","19325","19326","19327"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"15","bibliographicPageStart":"7","bibliographicVolumeNumber":"41","bibliographic_titles":[{"bibliographic_title":"Yonago Acta medica"},{"bibliographic_title":"Yonago Acta medica","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It has been recently suggested that nitric oxide (NO) plays an important role in modulating immune responses including helper T (Th) cell differentiation. To investigate the effect of NO on cytokine production in T cells, we examined in vitro the percentage of interferon (IFN)-γ and interleukin (IL)-4 producing cells by the intracellular cytokine staining method with flow cytometry. The percentage of IFN-γ and IL-4 producing cells reached maximal value 8 h after stimulation by phorbol 12-myristate 13-acetate (PMA) and ionomycin. When an NO donor, sodium nitroprusside (SNP), was co-incubated with PMA and ionomycin for 8 h, nitrite levels increased in a dose dependent manner for SNP (P = 0.007; Friedman test). The percentage of IFN-γ producing cells was diminished in consequence of the increasing doses of SNP (P = 0.002; Friedman test). While the percentage of IL-4 producing cells tended to be diminished by SNP, this difference, however, was statistically not significant (P = 0.062; Friedman test). Therefore, we have suggested that NO might affect Th cell differentiation through inhibition of Th1-cytokine production and might cause a Th2 cell predominant state.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tottori University Faculty of Medicine"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yonago Acta medica. 1998, 41(1), 7-15"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Yonago Acta medica　編集委員会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00892882","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468049","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_32":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Third Department of Internal Medicine, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Third Department of Internal Medicine, Faculty of Medicine, Tottori University"},{"subitem_text_value":"Third Department of Internal Medicine, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamasaki, Akira"},{"creatorName":"Yamasaki, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Tomita, Katsuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hitsuda, Yutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomita, Katsuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hitsuda, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2009-06-05"}],"displaytype":"detail","filename":"41_007-015.pdf","filesize":[{"value":"162.4 kB"}],"format":"application/pdf","licensefree":"Yonago Acta medica　編集委員会","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"41_007-015.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/5197/files/41_007-015.pdf"},"version_id":"98ed5213-15ee-4814-9b6c-57f14b747359"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"IFN-γ","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-4","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular cytokines","subitem_subject_scheme":"Other"},{"subitem_subject":"NO","subitem_subject_scheme":"Other"},{"subitem_subject":"sodium nitroprusside","subitem_subject_scheme":"Other"},{"subitem_subject":"IFN-γ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular cytokines","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NO","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sodium nitroprusside","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Effect of a Nitric Oxide Donor on Intracellular Cytokine Production in Normal Human Peripheral Lymphocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of a Nitric Oxide Donor on Intracellular Cytokine Production in Normal Human Peripheral Lymphocytes","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9","1096"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5197","relation_version_is_last":true,"title":["Effect of a Nitric Oxide Donor on Intracellular Cytokine Production in Normal Human Peripheral Lymphocytes"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-10-03T23:59:27.194933+00:00"}