@phdthesis{oai:repository.lib.tottori-u.ac.jp:00005865,
 author = {橋本, 祐樹 and Hashimoto, Yuki},
 month = {2018-05-31, 2018-05-31, 2018-05-31},
 note = {【Background】 Acute promyelocytic leukemia (APL) is a disease characterized by expression of promyelocytic leukemia?retinoic acid receptor α (PML-RARα) chimeric mRNA. Although APL is curable, early death due to hemorrhage is a major problem. Here, we report the development of a simple and rapid diagnostic method for APL based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). 【Methods】 An RT-LAMP primer set was designed to detect three types of PML-RARα mRNA in a single reaction. Serial dilutions of plasmid DNA containing bcr1, bcr2, or bcr3 PML-RARα sequences and RNA extracted from bone marrow aspirates of 6 patients with APL were used to compare the results of RT-LAMP and nested PCR assays. 【Results】 Plasmid DNA was amplified by RT-LAMP, for which the reaction time was > 4 h shorter and the lower detection limit was higher than for nested RTPCR. Six of 7 samples tested positive by both methods. 【Conclusion】 We developed an RT-LAMP assay for simple and rapid PML-RARα mRNA detection that may be clinically useful for point-of-care testing and APL diagnosis.},
 school = {鳥取大学},
 title = {前骨髄球性白血病レチノイン酸受容体αmRNAの簡便かつ迅速な検出のための逆転写ループ介在等温増幅法の構築},
 year = {},
 yomi = {ハシモト, ユウキ and ハシモト, ユウキ}
}