{"created":"2023-08-02T03:56:49.789466+00:00","id":5865,"links":{},"metadata":{"_buckets":{"deposit":"66925b90-4d19-4c41-b0f6-6cf6299a0f5d"},"_deposit":{"created_by":10,"id":"5865","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5865"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005865","sets":["1:9","2:13","24:58"]},"author_link":["40040","40041"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-01"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鳥取大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"15101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【Background】 Acute promyelocytic leukemia (APL) is a disease characterized by expression of promyelocytic leukemia?retinoic acid receptor α (PML-RARα) chimeric mRNA. Although APL is curable, early death due to hemorrhage is a major problem. Here, we report the development of a simple and rapid diagnostic method for APL based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). 【Methods】 An RT-LAMP primer set was designed to detect three types of PML-RARα mRNA in a single reaction. Serial dilutions of plasmid DNA containing bcr1, bcr2, or bcr3 PML-RARα sequences and RNA extracted from bone marrow aspirates of 6 patients with APL were used to compare the results of RT-LAMP and nested PCR assays. 【Results】 Plasmid DNA was amplified by RT-LAMP, for which the reaction time was > 4 h shorter and the lower detection limit was higher than for nested RTPCR. Six of 7 samples tested positive by both methods. 【Conclusion】 We developed an RT-LAMP assay for simple and rapid PML-RARα mRNA detection that may be clinically useful for point-of-care testing and APL diagnosis.","subitem_description_type":"Other"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第873号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"This research was published by Yonago Acta Medica: Yuki Hashimoto, Yuki Hatayama, Nao Kojima, Shota Morishita, Satoko Matsumoto, Yuzuru Hosoda, Ayako Hara and Toru Motokura.  Development of Reverse Transcription Loop-Mediated Isothermal Amplification for"}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.lib.tottori-u.ac.jp/yam/yam/yam59-4/59-4contents.html"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.lib.tottori-u.ac.jp/yam/yam/yam59-4/59-4contents.html","subitem_relation_type_select":"URI"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"注があるものを除き、この著作物は日本国著作権法により保護されています。"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"橋本, 祐樹"},{"creatorName":"ハシモト, ユウキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashimoto, Yuki","creatorNameLang":"en"},{"creatorName":"ハシモト, ユウキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-31"}],"displaytype":"detail","filename":"G11_15101A00873.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensefree":"注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文 : Full Text","objectType":"fulltext","url":"https://repository.lib.tottori-u.ac.jp/record/5865/files/G11_15101A00873.pdf"},"version_id":"06d9d4a4-aafa-4954-8f34-157865f45494"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-31"}],"displaytype":"detail","filename":"G11_15101A00873R.pdf","filesize":[{"value":"530.7 kB"}],"format":"application/pdf","licensefree":"注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨 : Summary","objectType":"abstract","url":"https://repository.lib.tottori-u.ac.jp/record/5865/files/G11_15101A00873R.pdf"},"version_id":"7b8ba249-90d5-4622-9a7b-0ccc59fa1b15"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-31"}],"displaytype":"detail","filename":"G11_15101A00873S.pdf","filesize":[{"value":"480.1 kB"}],"format":"application/pdf","licensefree":"注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果要旨 : Summary of Thesis Review","objectType":"abstract","url":"https://repository.lib.tottori-u.ac.jp/record/5865/files/G11_15101A00873S.pdf"},"version_id":"44c6ea30-5d58-4560-a862-94dda2150a89"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"acute promyelocytic leukemia","subitem_subject_scheme":"Other"},{"subitem_subject":"polymerase chain reaction","subitem_subject_scheme":"Other"},{"subitem_subject":"promyelocytic leukemia?retinoic acid receptor α","subitem_subject_scheme":"Other"},{"subitem_subject":"reverse transcription loop-mediated isothermal amplification","subitem_subject_scheme":"Other"},{"subitem_subject":"t(15;17) chromosome translocation","subitem_subject_scheme":"Other"},{"subitem_subject":"acute promyelocytic leukemia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"polymerase chain reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"promyelocytic leukemia?retinoic acid receptor α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"reverse transcription loop-mediated isothermal amplification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"t(15;17) chromosome translocation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis"}]},"item_title":"前骨髄球性白血病レチノイン酸受容体αmRNAの簡便かつ迅速な検出のための逆転写ループ介在等温増幅法の構築","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"前骨髄球性白血病レチノイン酸受容体αmRNAの簡便かつ迅速な検出のための逆転写ループ介在等温増幅法の構築","subitem_title_language":"ja"},{"subitem_title":"Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia?Retinoic Acid Receptor α mRNA","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"10","path":["13","9","58"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5865","relation_version_is_last":true,"title":["前骨髄球性白血病レチノイン酸受容体αmRNAの簡便かつ迅速な検出のための逆転写ループ介在等温増幅法の構築"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-27T08:40:49.164898+00:00"}