{"created":"2023-08-02T03:56:50.118084+00:00","id":5870,"links":{},"metadata":{"_buckets":{"deposit":"c345f984-7e92-4f12-8386-538904062a05"},"_deposit":{"created_by":10,"id":"5870","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"5870"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00005870","sets":["1:9","2:13","24:58"]},"author_link":["4574"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-01"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鳥取大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"15101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Sunitinib is a tyrosine kinase inhibitor that is used as the primary treatment in metastatic renal cell carcinoma (RCC). The main difficulty associated with its use is the development of drug resistance. In the present study, ACHN cells, a human renal cell carcinoma cell line, were used to establish sunitinib‑resistant (SR) cells. Microarray analysis and reverse transcription‑quantitative polymerase chain reaction revealed that miR‑194‑5p expression was significantly decreased in SR‑ACHN cells when compared with that observed in ACHN cells (P<0.05). Transfection of miR‑194‑5p, though not with negative control miR, in SR‑ACHN cells could significantly inhibit cell proliferation following sunitinib treatment (2.5‑40 µM; P<0.05). Western blotting demonstrated that the expression of lysosome‑associated membrane protein‑2 (LAMP‑2), which attenuates the anti‑proliferative effect of sunitinib, was significantly higher in SR‑ACHN than in ACHN cells (P<0.01). In addition, LAMP‑2 expression was suppressed by miR‑194‑5p transfection in SR‑ACHN cells. These data suggested that miR‑194‑5p downregulation may be associated with sunitinib resistance via the induction of LAMP‑2 expression in human RCC.","subitem_description_type":"Other"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第878号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"This research was published by Spandidos Publications: Yumioka, T., Osaki, M., Sasaki, R., Yamaguchi, N., Onuma, K., Iwamoto, H. ... Okada, F. (2018). Lysosome-associated membrane protein 2 (LAMP-2) expression induced by miR-194-5p downregulation contribu"}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.spandidos-publications.com/10.3892/ol.2017.7423"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.spandidos-publications.com/10.3892/ol.2017.7423","subitem_relation_type_select":"URI"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Yumioka et al. This is an open access article distributed under the terms of Creative Commons Attribution License: CC-BY-NC-ND(https://creativecommons.org/licenses/by-nc-nd/4.0/)."}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"弓岡, 徹也"},{"creatorName":"ユミオカ, テツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Yumioka, Tetsuya","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-31"}],"displaytype":"detail","filename":"G11_15101A00878.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensefree":"© Yumioka et al. This is an open access article distributed under the terms of Creative Commons Attribution License: CC-BY-NC-ND(https://creativecommons.org/licenses/by-nc-nd/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文 : Full Text","objectType":"fulltext","url":"https://repository.lib.tottori-u.ac.jp/record/5870/files/G11_15101A00878.pdf"},"version_id":"d10a0c16-7e27-4fb1-b03f-a38fd74fec8c"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-31"}],"displaytype":"detail","filename":"G11_15101A00878R.pdf","filesize":[{"value":"523.5 kB"}],"format":"application/pdf","licensefree":"注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨 : Summary","objectType":"abstract","url":"https://repository.lib.tottori-u.ac.jp/record/5870/files/G11_15101A00878R.pdf"},"version_id":"192c8b15-0557-4a28-8d54-95a7d23dcaff"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-31"}],"displaytype":"detail","filename":"G11_15101A00878S.pdf","filesize":[{"value":"478.6 kB"}],"format":"application/pdf","licensefree":"注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果要旨 : Summary of Thesis Review","objectType":"abstract","url":"https://repository.lib.tottori-u.ac.jp/record/5870/files/G11_15101A00878S.pdf"},"version_id":"11e739a1-86b9-4d59-8b88-d62e169c78fc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"microRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"renal cell carcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"sunitinib","subitem_subject_scheme":"Other"},{"subitem_subject":"resistance","subitem_subject_scheme":"Other"},{"subitem_subject":"lysosome?associated membrane protein 2","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"renal cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sunitinib","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lysosome?associated membrane protein 2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis"}]},"item_title":"miR-194-5pの低下により誘発されるリソソーム関連膜タンパク2（LAMP-2）の発現は、ヒト腎細胞癌細胞におけるスニチニブ抵抗性に寄与する","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"miR-194-5pの低下により誘発されるリソソーム関連膜タンパク2（LAMP-2）の発現は、ヒト腎細胞癌細胞におけるスニチニブ抵抗性に寄与する","subitem_title_language":"ja"},{"subitem_title":"Lysosome-associated membrane protein 2 (LAMP-2) expression induced by miR-194-5p downregulation contributes to sunitinib resistance in human renal cell carcinoma cells","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"10","path":["13","9","58"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"5870","relation_version_is_last":true,"title":["miR-194-5pの低下により誘発されるリソソーム関連膜タンパク2（LAMP-2）の発現は、ヒト腎細胞癌細胞におけるスニチニブ抵抗性に寄与する"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-27T08:41:04.717099+00:00"}