{"created":"2023-08-02T03:57:58.746847+00:00","id":7097,"links":{},"metadata":{"_buckets":{"deposit":"ed109fc7-6449-42aa-b72c-c44cf2fe840d"},"_deposit":{"created_by":10,"id":"7097","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7097"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007097","sets":["1:10","2:12"]},"author_link":["26246","4648","4673","4763","26247","26248"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"APPLIED SCIENCES"},{"bibliographic_title":"APPLIED SCIENCES","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Messenger RNA (mRNA) drugs have attracted considerable attention as promising tools with many therapeutic applications. The efficient delivery of mRNA drugs using non-viral materials is currently being explored. We demonstrate a novel concept where mCherry mRNA bearing a poly(A) tail is encapsulated into capsids co-assembled from viral β-annulus peptides bearing a 20-mer oligothymine (dT20) at the N-terminus and unmodified peptides via hybridization of dT20 and poly(A). Dynamic light scattering measurements and transmission electron microscopy images of the mRNA-encapsulated capsids show the formation of spherical assemblies of approximately 50 nm. The encapsulated mRNA shows remarkable ribonuclease resistance. Further, modification by a cell-penetrating peptide (His16) on the capsid enables the intracellular expression of mCherry of encapsulated mRNA.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/app10228004","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Nakamura Yoko, Sato Yuki, Inaba Hiroshi, et al. Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate and the Poly(A) Tail of mRNA. APPLIED SCIENCES-BASEL. 2020. 10(22). doi:10.3390/app10228004"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.mdpi.com/2076-3417/10/22/8004"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/2076-3417/10/22/8004","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"20763417"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Bioresources Science, Graduate School of Agricultural Sciences, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ナカムラ, ヨウコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"稲葉, 央"},{"creatorName":"イナバ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Inaba, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"岩崎, 崇"},{"creatorName":"イワサキ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Iwasaki, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"松浦, 和則"},{"creatorName":"マツウラ, カズノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsuura, Kazunori","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nakamura, Yoko","creatorNameLang":"en"},{"creatorName":"ナカムラ, ヨウコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"as10(22)_app10228004.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensefree":"(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"as10(22)_app10228004.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7097/files/as10(22)_app10228004.pdf"},"version_id":"70a8f6a4-c1ae-4a04-a448-d6a535c0faa6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"mRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"poly(A) tail","subitem_subject_scheme":"Other"},{"subitem_subject":"artificial viral capsid","subitem_subject_scheme":"Other"},{"subitem_subject":"encapsulation","subitem_subject_scheme":"Other"},{"subitem_subject":"nanocapsule","subitem_subject_scheme":"Other"},{"subitem_subject":"self-assembly","subitem_subject_scheme":"Other"},{"subitem_subject":"β-annulus peptide","subitem_subject_scheme":"Other"},{"subitem_subject":"peptide-DNA conjugate","subitem_subject_scheme":"Other"},{"subitem_subject":"mRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"poly(A) tail","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"artificial viral capsid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"encapsulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nanocapsule","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"self-assembly","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"β-annulus peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"peptide-DNA conjugate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate and the Poly(A) Tail of mRNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate and the Poly(A) Tail of mRNA","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","10"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-06"},"publish_date":"2022-07-06","publish_status":"0","recid":"7097","relation_version_is_last":true,"title":["Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate and the Poly(A) Tail of mRNA"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:33:01.230254+00:00"}