{"created":"2023-08-02T03:57:59.487463+00:00","id":7110,"links":{},"metadata":{"_buckets":{"deposit":"44e1c343-f8da-4724-a622-087c4a241e61"},"_deposit":{"created_by":10,"id":"7110","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7110"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007110","sets":["1:9","2:12"]},"author_link":["26297","4929","4904","4750","26298","26299","26300","26301","26302"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Biology Open"},{"bibliographic_title":"Biology Open","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Microtubule-associated protein A1/B1-light chain 3 (LC3)-associated phagocytosis (LAP) is a type of non-canonical autophagy that regulates phagosome maturation in macrophages. However, the role and regulatory mechanism of LAP remain largely unknown. Recently, the membrane occupation and recognition nexus repeat-containing-2 (MORN2) was identified as a key component of LAP for the efficient formation of LC3-recruiting phagosomes. To characterize MORN2 and elucidate its function in LAP, we established a MORN2-overexpressing macrophage line. At a steady state, MORN2 was partially cleaved by the ubiquitin-proteasome system. MORN2 overexpression promoted not only LC3-II production but also LAP phagosome (LAPosome) acidification during Escherichia coli uptake. Furthermore, the formation of LAPosomes containing the yeast cell wall component zymosan was enhanced in MORN2-overexpressing cells and depended on reactive oxygen species (ROS). Finally, MORN2-mediated LAP was regulated by plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) such as SNAP-23 and syntaxin 11. Taken together, these findings demonstrate that MORN2, whose expression is downregulated via proteasomal digestion, is a limiting factor for LAP, and that membrane trafficking by SNARE proteins is involved in MORN2-mediated LAP.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Company of Biologists"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1242/bio.051029","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Morita Maya, Kajiye Mayu, Sakurai Chiye, et al. Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages. Biology open. 2020. 9(6). doi: 10.1242/bio.051029"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://journals.biologists.com/bio/article/9/6/bio051029/222866"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://journals.biologists.com/bio/article/9/6/bio051029/222866","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and repr"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20466390","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田, 真矢"},{"creatorName":"モリタ, マヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"26297","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"櫻井, 千恵"},{"creatorName":"サクライ, チエ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakurai, Chiye","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4929","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10589724","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10589724"},{"nameIdentifier":"100001302","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001302_ja.html"}]},{"creatorNames":[{"creatorName":"堀, 直裕"},{"creatorName":"ホリ, ナオヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Hori, Naohiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4904","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80263466","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80263466"},{"nameIdentifier":"100000245","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000245_ja.html"}]},{"creatorNames":[{"creatorName":"初沢, 清隆"},{"creatorName":"ハツザワ, キヨタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Hatsuzawa, Kiyotaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4750","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20256655","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20256655"},{"nameIdentifier":"100001086","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001086_ja.html"}]},{"creatorNames":[{"creatorName":"Morita, Maya","creatorNameLang":"en"},{"creatorName":"モリタ, マヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"26298","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kajiye, Mayu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26299","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubo, Shuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26300","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Miki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26301","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kinoshita, Daiki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26302","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"bo9(6)_bio_051029.pdf","filesize":[{"value":"5.5 MB"}],"format":"application/pdf","licensefree":"© 2020. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and repr","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bo9(6)_bio_051029.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7110/files/bo9(6)_bio_051029.pdf"},"version_id":"230cd8ea-51fb-47b1-af8c-208b3448018b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LC3-associated phagocytosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Membrane trafficking","subitem_subject_scheme":"Other"},{"subitem_subject":"MORN2","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-canonical autophagy","subitem_subject_scheme":"Other"},{"subitem_subject":"Phagosome maturation","subitem_subject_scheme":"Other"},{"subitem_subject":"SNARE protein","subitem_subject_scheme":"Other"},{"subitem_subject":"LC3-associated phagocytosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Membrane trafficking","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MORN2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-canonical autophagy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phagosome maturation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SNARE protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-11-25"},"publish_date":"2021-11-25","publish_status":"0","recid":"7110","relation_version_is_last":true,"title":["Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:33:47.333401+00:00"}