@article{oai:repository.lib.tottori-u.ac.jp:00007161,
 author = {角, 啓佑 and 大倉, 毅 and Okura, Tsuyoshi and 藤岡, 洋平 and Fujioka, Yohei and 加藤, 雅彦 and Kato, Masahiko and 今村, 武史 and Imamura, Takeshi and 谷口, 晋一 and Taniguchi, Shin-ichi and 山本, 一博 and Yamamoto, Kazuhiro and Sumi, Keisuke},
 journal = {DIABETOLOGY & METABOLIC SYNDROME, DIABETOLOGY & METABOLIC SYNDROME},
 month = {Jun},
 note = {Background: In mitochondrial diabetes, apoptosis of β-cells caused by mitochondrial stress plays an important role in impaired insulin secretion. Several studies have reported that coenzyme Q10 (CoQ10) has therapeutic effects on mitochondrial diabetes, but no reports have examined the fundamental effectiveness or mechanism of CoQ10 in mitochondrial diabetes. We previously reported in a Japanese article that CoQ10 has protective effects on pancreatic β-cells against mitochondrial stress using mouse pancreatic β-cell line MIN6 and staurosporine (STS). Here, we report that CoQ10 protects MIN6 cells against apoptosis caused by STS and describe the more detailed apoptotic cascade. Methods: Apoptosis of MIN6 cells was induced by 0.5 μM STS treatment for specific periods with or without 30 μM CoQ10. The apoptosis cascade in MIN6 cells was then investigated using WST-8 assays, annexin-V staining, western blotting, and DNA degradation analysis. Results: Sixteen hours of 0.5 μM STS treatment led to 47% cell viability, but pretreatment with 30 μM CoQ10 resulted in significantly higher viability of 76% (P < 0.01). CoQ10 also prevented translocation of phosphatidylserine from the inner leaflet to the outer leaflet of the cell membrane. CoQ10 prevented cytochrome c release from mitochondria and activation of caspase-3. Conclusion: We concluded that CoQ10 protects pancreatic β-cells through anti-apoptotic effects against STS treatment.},
 title = {Coenzyme Q10 suppresses apoptosis of mouse pancreatic beta-cell line MIN6},
 volume = {10},
 year = {2018},
 yomi = {スミ, ケイスケ and オオクラ, ツヨシ and フジオカ, ヨウヘイ and カトウ, マサヒコ and イマムラ, タケシ and タニグチ, シンイチ and ヤマモト, カズヒロ and スミ, ケイスケ}
}