{"created":"2023-08-02T03:58:02.090745+00:00","id":7161,"links":{},"metadata":{"_buckets":{"deposit":"be3df936-b6ad-49f5-a3a3-d8123ef60722"},"_deposit":{"created_by":10,"id":"7161","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7161"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007161","sets":["1:9","2:12"]},"author_link":["26514","2790","349","1364","4712","3805","4288","26515"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-14","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"DIABETOLOGY & METABOLIC SYNDROME"},{"bibliographic_title":"DIABETOLOGY & METABOLIC SYNDROME","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: In mitochondrial diabetes, apoptosis of β-cells caused by mitochondrial stress plays an important role in impaired insulin secretion. Several studies have reported that coenzyme Q10 (CoQ10) has therapeutic effects on mitochondrial diabetes, but no reports have examined the fundamental effectiveness or mechanism of CoQ10 in mitochondrial diabetes. We previously reported in a Japanese article that CoQ10 has protective effects on pancreatic β-cells against mitochondrial stress using mouse pancreatic β-cell line MIN6 and staurosporine (STS). Here, we report that CoQ10 protects MIN6 cells against apoptosis caused by STS and describe the more detailed apoptotic cascade. Methods: Apoptosis of MIN6 cells was induced by 0.5 μM STS treatment for specific periods with or without 30 μM CoQ10. The apoptosis cascade in MIN6 cells was then investigated using WST-8 assays, annexin-V staining, western blotting, and DNA degradation analysis. Results: Sixteen hours of 0.5 μM STS treatment led to 47% cell viability, but pretreatment with 30 μM CoQ10 resulted in significantly higher viability of 76% (P < 0.01). CoQ10 also prevented translocation of phosphatidylserine from the inner leaflet to the outer leaflet of the cell membrane. CoQ10 prevented cytochrome c release from mitochondria and activation of caspase-3. Conclusion: We concluded that CoQ10 protects pancreatic β-cells through anti-apoptotic effects against STS treatment.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd."}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s13098-018-0351-4","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Sumi Keisuke, Okura Tsuyoshi, Fujioka Youhei, et al. Coenzyme Q10 suppresses apoptosis of mouse pancreatic beta-cell line MIN6. DIABETOLOGY & METABOLIC SYNDROME. 2018. 10. doi:10.1186/s13098-018-0351-4"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://dmsjournal.biomedcentral.com/articles/10.1186/s13098-018-0351-4"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dmsjournal.biomedcentral.com/articles/10.1186/s13098-018-0351-4","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"17585996","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Pharmacology, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Regional Medicine, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"角, 啓佑"},{"creatorName":"スミ, ケイスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"26514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大倉, 毅"},{"creatorName":"オオクラ, ツヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"Okura, 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マサヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Masahiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1364","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40362884","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40362884"},{"nameIdentifier":"100000127","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000127_ja.html"}]},{"creatorNames":[{"creatorName":"今村, 武史"},{"creatorName":"イマムラ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"Imamura, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4712","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00552093","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00552093"},{"nameIdentifier":"100001754","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001754_ja.html"}]},{"creatorNames":[{"creatorName":"谷口, 晋一"},{"creatorName":"タニグチ, シンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Taniguchi, Shin-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3805","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30304207","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30304207"},{"nameIdentifier":"100000183","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000183_ja.html"}]},{"creatorNames":[{"creatorName":"山本, 一博"},{"creatorName":"ヤマモト, カズヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamamoto, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4288","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90303966","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90303966"},{"nameIdentifier":"100000280","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000280_ja.html"}]},{"creatorNames":[{"creatorName":"Sumi, Keisuke","creatorNameLang":"en"},{"creatorName":"スミ, ケイスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"26515","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"dms10_s13098-018-0351-4.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensefree":"© The Author(s) 2018. 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