{"created":"2023-08-02T03:58:05.293892+00:00","id":7230,"links":{},"metadata":{"_buckets":{"deposit":"92827744-5a61-45af-ac1d-b5b01e37e3fb"},"_deposit":{"created_by":10,"id":"7230","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7230"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007230","sets":["1:9","2:12"]},"author_link":["4866","95","780","377","3368","26790","26791"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES"},{"bibliographic_title":"INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CD44, a cancer stem cell (CSC) marker, is required for maintaining CSC properties in hepatocellular carcinoma (HCC). Nuclear enriched abundant transcript 1 (NEAT1), a long noncoding RNA (lncRNA), is an oncogenic driver in HCC. In the present study, we investigated the significance of the NEAT1 gene in association with CD44 expression in liver CSCs of human HCC cell lines. The CSC properties were evaluated by spheroid culture, CSC marker expression, and sensitivity to anti-cancer drugs. The expression of both NEAT1 variant 1 (NEAT1v1) and variant 2 (NEAT1v2) as well as CD44 was significantly increased in the spheroid culture, compared with that in monolayer culture. Overexpression of Neat1v1, but not Neat1v2, enhanced the CSC properties, while knockout of the NEAT1 gene suppressed them. CD44 expression was increased by the overexpression of Neat1v1 and abrogated by NEAT1 knockout. The overexpression of NEAT1v1 restored the CSC properties and CD44 expression in NEAT1-knockout cells. NEAT1v1 expression in HCC tissues was correlated with poor prognosis and CD44 expression. These results suggest that NEAT1v1 is required for CD44 expression. To our surprise, NEAT1v1 also restored the CSC properties even in CD44-deficient cells, suggesting that NEAT1v1 maintains the properties of CSCs in a CD44-independent manner.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms21061927","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Koyama Shigemi, Tsuchiya Hiroyuki, Amisaki Masataka, et al. NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020. 21(6). doi:10.3390/ijms21061927"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.mdpi.com/1422-0067/21/6/1927"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/1422-0067/21/6/1927","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"14220067"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Radiology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"土谷, 博之"},{"creatorName":"ツチヤ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsuchiya, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"網崎, 正孝"},{"creatorName":"アミサキ, マサタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Amisaki, Masataka","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"本城, 総一郎"},{"creatorName":"ホンジョウ, ソウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Honjo, Soichiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"藤原, 義之"},{"creatorName":"フジワラ, ヨシユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujiwara, Yoshiyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"汐田, 剛史"},{"creatorName":"シオタ, ゴウシ","creatorNameLang":"ja-Kana"},{"creatorName":"Shiota, Goshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Koyama, Shigemi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakaguchi, Hiromi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"ijms21_ijms21061927.pdf","filesize":[{"value":"6.8 MB"}],"format":"application/pdf","licensefree":"© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ijms21_ijms21061927.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7230/files/ijms21_ijms21061927.pdf"},"version_id":"ac97fe46-286c-4344-a4c1-8ee500abd254"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cancer stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"NEAT1","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocellular carcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"CD44","subitem_subject_scheme":"Other"},{"subitem_subject":"spheroid","subitem_subject_scheme":"Other"},{"subitem_subject":"cancer stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NEAT1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocellular carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CD44","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"spheroid","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-09-08"},"publish_date":"2021-09-08","publish_status":"0","recid":"7230","relation_version_is_last":true,"title":["NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:40:29.461615+00:00"}