@article{oai:repository.lib.tottori-u.ac.jp:00007242,
 author = {櫻井, 千恵 and Sakurai, Chiye and 堀, 直裕 and Hori, Naohiro and 初沢, 清隆 and Hatsuzawa, Kiyotaka and Morita, Maya and Sawaki, Kazumasa and Kinoshita, Daiki},
 issue = {5},
 journal = {JOURNAL OF BIOCHEMISTRY, JOURNAL OF BIOCHEMISTRY},
 month = {Nov},
 note = {Phagosome formation and maturation are essential innate immune mechanisms to engulf and digest foreign particles. To analyze these processes quantitatively, we established a specific Escherichia coli probe expressing a tandem fluorescent protein, comprising glutathione S-transferase fused with monomeric Cherry (mCherry) and monomeric Venus (mVenus). We demonstrated that mVenus was more susceptible to bleaching in an acidic environment than mCherry, and that the mVenus:mCherry fluorescence intensity ratio can be used to monitor phagosomal pH changes during maturation. Using this probe, we revealed that synaptosomal-associated protein of 23 kDa, a plasma membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein, actively regulated phagocytosis of E. coli and subsequent phagosome maturation in macrophages. Our results indicated that this probe has the potential to be a powerful tool in understanding the molecular mechanisms of phagosome formation and maturation.},
 pages = {309--316},
 title = {Quantitative analysis of phagosome formation and maturation using an Escherichia coli probe expressing a tandem fluorescent protein},
 volume = {162},
 year = {2017},
 yomi = {サクライ, チエ and ホリ, ナオヒロ and ハツザワ, キヨタカ}
}