{"created":"2023-08-02T03:58:05.905001+00:00","id":7242,"links":{},"metadata":{"_buckets":{"deposit":"26f3d06f-930e-48d5-9b86-afa388b4095d"},"_deposit":{"created_by":10,"id":"7242","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7242"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007242","sets":["1:9","2:12"]},"author_link":["4929","4904","4750","26869","26870","26871"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"316","bibliographicPageStart":"309","bibliographicVolumeNumber":"162","bibliographic_titles":[{"bibliographic_title":"JOURNAL OF BIOCHEMISTRY"},{"bibliographic_title":"JOURNAL OF BIOCHEMISTRY","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phagosome formation and maturation are essential innate immune mechanisms to engulf and digest foreign particles. To analyze these processes quantitatively, we established a specific Escherichia coli probe expressing a tandem fluorescent protein, comprising glutathione S-transferase fused with monomeric Cherry (mCherry) and monomeric Venus (mVenus). We demonstrated that mVenus was more susceptible to bleaching in an acidic environment than mCherry, and that the mVenus:mCherry fluorescence intensity ratio can be used to monitor phagosomal pH changes during maturation. Using this probe, we revealed that synaptosomal-associated protein of 23 kDa, a plasma membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein, actively regulated phagocytosis of E. coli and subsequent phagosome maturation in macrophages. Our results indicated that this probe has the potential to be a powerful tool in understanding the molecular mechanisms of phagosome formation and maturation.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvx034","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Morita Maya, Sawaki Kazumasa, Kinoshita Daiki, et al. Quantitative analysis of phagosome formation and maturation using an Escherichia coli probe expressing a tandem fluorescent protein. JOURNAL OF BIOCHEMISTRY. 2017. 162(5). 309-316. doi:10.1093/jb/mvx03"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://academic.oup.com/jb/article/162/5/309/3858071"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://academic.oup.com/jb/article/162/5/309/3858071","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of biochemistry following peer review. The version of record Morita Maya, Sawaki Kazumasa, Kinoshita Daiki, et al. 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The version of record Morita Maya, Sawaki Kazumasa, Kinoshita Daiki, et al. 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