@article{oai:repository.lib.tottori-u.ac.jp:00007247,
 author = {原田, 真吾 and Harada, Shingo and 中村, 嘉伸 and Nakamura, Yoshinobu and 白谷, 卓 and Shiraya, Suguru and 藤原, 義和 and Fujiwara, Yoshikazu and 岸本, 祐一郎 and Kishimoto, Yuichiro and 大月, 優貴 and Otsuki, Yuki and 岸本, 諭 and Kishimoto, Satoru and 山本, 康孝 and Yamamoto, Yasutaka and 久留, 一郎 and Hisatome, Ichiro and 西村, 元延 and Nishimura, Motonobu and Onohara, Takeshi},
 journal = {JOURNAL OF CARDIOTHORACIC SURGERY, JOURNAL OF CARDIOTHORACIC SURGERY},
 month = {Aug},
 note = {Background: We examined whether a vascular smooth muscle cell (SMC) sheet is effective in the treatment of a rat myocardial infarction (MI) model. Methods: We examined the effect of SMC sheet on the cardiac function and cardiac remodeling in a rat MI model in comparison with their effect of dermal fibroblast (DFB) sheet in vivo. Furthermore, we estimated the apoptosis and secretion of angiogenic factor of SMC under hypoxic condition in comparison with DFB. Seven days after MI, monolayer cell sheets were transplanted on the infarcted area (SMC transplantation group, SMC-Tx; DFB transplantation group, DFB-Tx; no cell sheet transplantation group, Untreated; neither MI nor cell sheet transplantation group, Sham). We evaluated cardiac function by echocardiogram, degree of cardiac remodeling by histological examination, and secretion of angiogenic growth factor by enzyme immunoassay. Results: Twenty-eight days after transplantation, SMC-Tx showed the following characteristics compared with the other groups: 1) significantly greater fractional area shortening (SMC-Tx, 32.3 ± 2.1 %; DFB-Tx, 23.3 ± 2.1 %; untreated, 25.1 ± 2.6 %), 2) suppressed left ventricular dilation, smaller scar expansion, and preserved wall thickness of the area at risk and the posterior wall, 3) decreased fibrosis, preserved myocardium in the scar area, and greater number of arterioles in border-zone, 4) tight attachment of SMC sheets on the scarred myocardium, and less apoptotic cell death. In in vitro experiments, SMCs secreted higher amounts of basic fibroblast growth factor (SMC, 157.7 ± 6.4 pg/ml; DFB, 3.1 ± 1.0 pg/ml), and showed less apoptotic cell death under hypoxia. Conclusions: Our results illustrate that transplantation of SMC sheets inhibited the progression of cardiac remodeling and improve cardiac function. These beneficial effects may be due to superior SMC survival.},
 title = {Smooth muscle cell sheet transplantation preserve cardiac function and minimize cardiac remodeling in a rat myocardial infarction model},
 volume = {11},
 year = {2016},
 yomi = {ハラダ, シンゴ and ナカムラ, ヨシノブ and シラヤ, スグル and フジワラ, ヨシカズ and キシモト, ユウイチロウ and オツキ, ユキ and キシモト, サトル and ヤマモト, ヤスタカ and ヒサトメ, イチロ and ニシムラ, モトノブ}
}