{"created":"2023-08-02T03:58:09.066738+00:00","id":7300,"links":{},"metadata":{"_buckets":{"deposit":"36a30826-0e9e-4937-a8d4-6cec2c3d9c6b"},"_deposit":{"created_by":10,"id":"7300","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7300"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007300","sets":["1:7","2:12"]},"author_link":["4934","187","4792","4863","27092","27093","27094","27095","27096","27097","27098","27099"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageStart":"334","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"METABOLITES"},{"bibliographic_title":"METABOLITES","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ascorbate (AsA) is required as a cofactor and is widely distributed in plants and animals. Recently, it has been suggested that the nematode Caenorhabditis elegans also synthesizes AsA. However, its biosynthetic pathway is still unknown. To further understand AsA biosynthesis in C. elegans, we analyzed the incorporation of the 13C atom into AsA using gas chromatography-mass spectrometry (GC-MS) in worms fed with D-Glc (1-13C)-labeled Escherichia coli. GC-MS analysis revealed that AsA biosynthesis in C. elegans, similarly to that in mammalian systems, involves carbon skeleton rearrangement. The addition of L-gulono-1,4-lactone, an AsA precursor in the mammalian pathway, significantly increased AsA level in C. elegans, whereas the addition of L-galactono-1,4-lactone, an AsA precursor in the plant and Euglena pathway, did not affect AsA level. The suppression of E03H4.3 (an ortholog of gluconolactonase) or the deficiency of F54D5.12 (an ortholog of L-gulono-1,4-lactone oxidase) significantly decreased AsA level in C. elegans. Although N2- and AsA-deficient F54D5.12 knockout mutant worm (tm6671) morphologies and the ratio of collagen to non-collagen protein did not show any significant differences, the mutant worms exhibited increased malondialdehyde levels and reduced lifespan compared with the N2 worms. In conclusion, our findings indicate that the AsA biosynthetic pathway is similar in C. elegans and mammals.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/metabo10080334","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Yabuta Yukinori, Nagata Ryuta, Aoki Yuka, et al. L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans. METABOLITES. 2020. 10(8). doi:10.3390/metabo10080334"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.3390/metabo10080334"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/metabo10080334","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"22181989"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"The United Graduate School of Agricultural Sciences, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Electronic and Organic Material Laboratory, Tottori Institute of Industrial Technology"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藪田, 行哲"},{"creatorName":"ヤブタ, ユキノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Yabuta, Yukinori","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"美藤, 友博"},{"creatorName":"ビトウ, トモヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Bito, Tomohiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"石原, 亨"},{"creatorName":"イシハラ, アツシ","creatorNameLang":"ja-Kana"},{"creatorName":"Ishihara, Atsushi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"渡邉, 文雄"},{"creatorName":"ワタナベ, フミオ","creatorNameLang":"ja-Kana"},{"creatorName":"Watanabe, Fumio","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nagata, Ryuta","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aoki, Yuka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kariya, Ayumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wada, Kousuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagimoto, Ayako","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hara, Hiroka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Naho","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Shinichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"mtb10(8)_metabo10080334.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","licensefree":"(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"mtb10(8)_metabo10080334.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7300/files/mtb10(8)_metabo10080334.pdf"},"version_id":"fb0b0605-2a04-44b9-bc87-d27f440ccaf3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"L-ascorbate (AsA)","subitem_subject_scheme":"Other"},{"subitem_subject":"antioxidant","subitem_subject_scheme":"Other"},{"subitem_subject":"biosynthetic pathway","subitem_subject_scheme":"Other"},{"subitem_subject":"Caenorhabditis elegans","subitem_subject_scheme":"Other"},{"subitem_subject":"reactive oxygen species","subitem_subject_scheme":"Other"},{"subitem_subject":"redox","subitem_subject_scheme":"Other"},{"subitem_subject":"L-ascorbate (AsA)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"antioxidant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biosynthetic pathway","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Caenorhabditis elegans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"reactive oxygen species","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"redox","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","7"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-08-26"},"publish_date":"2022-08-26","publish_status":"0","recid":"7300","relation_version_is_last":true,"title":["L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:44:28.666722+00:00"}