{"created":"2023-08-02T03:58:09.491274+00:00","id":7309,"links":{},"metadata":{"_buckets":{"deposit":"5612ff1b-6221-42ae-a5f6-cf722a6997d7"},"_deposit":{"created_by":10,"id":"7309","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7309"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007309","sets":["1:9","2:12"]},"author_link":["27151","4929","4904","4750","27152","27153","27154"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-04-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"1128","bibliographicPageStart":"1051","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"MOLECULAR BIOLOGY OF THE CELL"},{"bibliographic_title":"MOLECULAR BIOLOGY OF THE CELL","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Syntaxin 11 (stx11) is a soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) that is selectively expressed in immune cells; however, its precise role in macrophages is unclear. We showed that stx11 knockdown reduces the phagocytosis of Escherichia coli in interferon-γ–activated macrophages. stx11 knockdown decreased Toll-like receptor 4 (TLR4) localization on the plasma membrane without affecting total expression. Plasma membrane–localized TLR4 was primarily endocytosed within 1 h by lipopolysaccharide (LPS) stimulation and gradually relocalized 4 h after removal of LPS. This relocalization was significantly impaired by stx11 knockdown. The lack of TLR4 transport to the plasma membrane is presumably related to TLR4 degradation in acidic endosomal organelles. Additionally, an immunoprecipitation experiment suggested that stx11 interacts with SNAP-23, a plasma membrane–localized SNARE protein, whose depletion also inhibits TLR4 replenishment in LPS-stimulated cells. Using an intramolecular Förster resonance energy transfer (FRET) probe for SNAP-23, we showed that the high FRET efficiency caused by LPS stimulation is reduced by stx11 knockdown. These findings suggest that stx11 regulates the stimulus-dependent transport of TLR4 to the plasma membrane by cooperating with SNAP-23 in macrophages. Our results clarify the regulatory mechanisms underlying intracellular transport of TLR4 and have implications for microbial pathogenesis and immune responses.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Cell Biology"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1091/mbc.E18-10-0653","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Kinoshita, Daiki, Sakurai Chiye, Morita Maya et al. Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages. Molecular biology of the cell, 2019, 30(9), 1085-1097."}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.molbiolcell.org/doi/10.1091/mbc.E18-10-0653"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.molbiolcell.org/doi/10.1091/mbc.E18-10-0653","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019 Kinoshita et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10591524","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"19394586"}]},"item_10001_text_33":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木下, 大生"},{"creatorName":"キノシタ, ダイキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"櫻井, 千恵"},{"creatorName":"サクライ, チエ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakurai, Chiye","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"堀, 直裕"},{"creatorName":"ホリ, ナオヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Hori, Naohiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"初沢, 清隆"},{"creatorName":"ハツザワ, キヨタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Hatsuzawa, Kiyotaka","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kinoshita, Daiki","creatorNameLang":"en"},{"creatorName":"キノシタ, ダイキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Maya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsunematsu, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"mbc30(9)_1085.pdf","filesize":[{"value":"5.7 MB"}],"format":"application/pdf","licensefree":"© 2019 Kinoshita et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"mbc30(9)_1085.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7309/files/mbc30(9)_1085.pdf"},"version_id":"85db61ba-4c1b-4504-bccf-c542ddc22b6e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","9"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-11-25"},"publish_date":"2021-11-25","publish_status":"0","recid":"7309","relation_version_is_last":true,"title":["Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:44:59.079802+00:00"}