{"created":"2023-08-02T03:58:11.576196+00:00","id":7346,"links":{},"metadata":{"_buckets":{"deposit":"85013dcd-68b1-463f-b37c-f83283ee67f1"},"_deposit":{"created_by":10,"id":"7346","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7346"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007346","sets":["1:10","2:12"]},"author_link":["4763","4648","27342","27343"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"PROCESSES"},{"bibliographic_title":"PROCESSES","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Artificial construction of spherical protein assemblies has attracted considerable attention due to its potential use in nanocontainers, nanocarriers, and nanoreactors. In this work, we demonstrate a novel strategy to construct peptide nanocapsules (artificial viral capsids) decorated with enzymes via interactions between His-tag and Ni-NTA. A β-annulus peptide derived from the tomato bushy stunt virus was modified with Ni-NTA at the C-terminus, which is directed toward the exterior surface of the artificial viral capsid. The β-annulus peptide bearing Ni-NTA at the C-terminus self-assembled into capsids of about 50 nm in diameter. The Ni-NTA-displayed capsids were complexed with recombinant horseradish peroxidase (HRP) with a C-terminal His-tag which was expressed in Escherichia coli. The β-annulus peptide-HRP complex formed spherical assemblies whose sizes were 30–90 nm, with the ζ-potential revealing that the HRP was decorated on the outer surface of the capsid.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/pr8111455","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Matsuura Kazunori, Shiomi Yuriko, Mizuta Toshihumi, et al. Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA. PROCESSES. 2020. 8(11). doi:10.3390/pr8111455"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.mdpi.com/2227-9717/8/11/1455"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/2227-9717/8/11/1455","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)."}]},"item_10001_text_26":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_text_value":"22279717"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Technical Department, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松浦, 和則"},{"creatorName":"マツウラ, カズノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsuura, Kazunori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4763","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60283389","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60283389"},{"nameIdentifier":"100000881","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000881_ja.html"}]},{"creatorNames":[{"creatorName":"稲葉, 央"},{"creatorName":"イナバ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Inaba, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4648","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00778011","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00778011"},{"nameIdentifier":"100001643","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001643_ja.html"}]},{"creatorNames":[{"creatorName":"Shiomi, Yuriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27342","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizuta, Toshihumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27343","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"prc8(11)_pr8111455.pdf","filesize":[{"value":"3.4 MB"}],"format":"application/pdf","licensefree":"(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"prc8(11)_pr8111455.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7346/files/prc8(11)_pr8111455.pdf"},"version_id":"a1ab7b57-7120-485a-8a66-6bfd04577ed3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"artificial viral capsid","subitem_subject_scheme":"Other"},{"subitem_subject":"self-assembly","subitem_subject_scheme":"Other"},{"subitem_subject":"β-annulus peptide","subitem_subject_scheme":"Other"},{"subitem_subject":"horseradish peroxidase","subitem_subject_scheme":"Other"},{"subitem_subject":"nanocapsule","subitem_subject_scheme":"Other"},{"subitem_subject":"surface decoration","subitem_subject_scheme":"Other"},{"subitem_subject":"artificial viral capsid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"self-assembly","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"β-annulus peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"horseradish peroxidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nanocapsule","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"surface decoration","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","10"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-06"},"publish_date":"2022-07-06","publish_status":"0","recid":"7346","relation_version_is_last":true,"title":["Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:47:00.424803+00:00"}