{"created":"2023-08-02T03:58:12.729336+00:00","id":7369,"links":{},"metadata":{"_buckets":{"deposit":"019a4d61-f78e-4ef0-8ebb-e703a4fce456"},"_deposit":{"created_by":10,"id":"7369","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7369"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007369","sets":["1:10","2:12"]},"author_link":["779","2225","1457","27412","27413"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-08-04","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In E. coli cells, rescue of non-native proteins and promotion of native state structure is assisted by the chaperonin GroEL. An important key to this activity lies in the structure of the apical domain of GroEL (GroEL-AD) (residue 191–376), which recognizes and binds non-native protein molecules through hydrophobic interactions. In this study, we investigated the effects of GroEL-AD on the aggregation of various client proteins (α-Synuclein, Aβ42, and GroES) that lead to the formation of distinct protein fibrils in vitro. We found that GroEL-AD effectively inhibited the fibril formation of these three proteins when added at concentrations above a critical threshold; the specific ratio differed for each client protein, reflecting the relative affinities. The effect of GroEL-AD in all three cases was to decrease the concentration of aggregate-forming unfolded client protein or its early intermediates in solution, thereby preventing aggregation and fibrillation. Binding affinity assays revealed some differences in the binding mechanisms of GroEL-AD toward each client. Our findings suggest a possible applicability of this minimal functioning derivative of the chaperonins (the “minichaperones”) as protein fibrillation modulators and detectors.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/srep31041","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Ojha Bimlesh, Fukui Naoya, Hongo Kunihiro, et al. Suppression of amyloid fibrils using the GroEL apical domain. Scientific Reports. 2016. 6. doi:10.1038/srep31041"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.nature.com/articles/srep31041"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/srep31041","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in th"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Department of Chemistry and Biotechnology, Graduate School of Engineering Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"本郷, 邦広"},{"creatorName":"ホンゴウ, クニヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Hongo, Kunihiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"779","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80335504","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80335504"},{"nameIdentifier":"100000563","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000563_ja.html"}]},{"creatorNames":[{"creatorName":"溝端, 知宏"},{"creatorName":"ミゾバタ, トモヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Mizobata, Tomohiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2225","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50263489","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50263489"},{"nameIdentifier":"100000572","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000572_ja.html"}]},{"creatorNames":[{"creatorName":"河田, 康志"},{"creatorName":"カワタ, ヤスシ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawata, Yasushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1457","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40177697","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40177697"},{"nameIdentifier":"100000496","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000496_ja.html"}]},{"creatorNames":[{"creatorName":"Ojha, Bimlesh","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27412","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukui, Naoya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27413","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"sr_srep31041.pdf","filesize":[{"value":"5.7 MB"}],"format":"application/pdf","licensefree":"(C) The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in th","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"sr_srep31041.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7369/files/sr_srep31041.pdf"},"version_id":"2ff45503-939d-4eee-a9e6-4722d1da0fe4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Suppression of amyloid fibrils using the GroEL apical domain","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Suppression of amyloid fibrils using the GroEL apical domain","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","10"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-30"},"publish_date":"2022-06-30","publish_status":"0","recid":"7369","relation_version_is_last":true,"title":["Suppression of amyloid fibrils using the GroEL apical domain"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:48:25.254791+00:00"}