{"created":"2023-08-02T03:58:12.817397+00:00","id":7371,"links":{},"metadata":{"_buckets":{"deposit":"668fca21-74d4-4818-bdd7-5144a714114f"},"_deposit":{"created_by":10,"id":"7371","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7371"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007371","sets":["1:11","2:12"]},"author_link":["4856","27432","27433","27434","27435"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10-31","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"16117","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS"},{"bibliographic_title":"SCIENTIFIC REPORTS","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Developing wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47 L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530 L. mollis SNP markers and the chromosome-specific SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-018-34545-x","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Edet Offiong U., Gorafi Yasir S. A., Cho Seong-woo, et al. Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines. SCIENTIFIC REPORTS. 2018. 8. doi:10.1038/s41598-018-34545-x"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.1038/s41598-018-34545-x"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41598-018-34545-x","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the o"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Arid Land Research Center, Tottori University / United Graduate School of Agricultural Sciences, Tottori University"},{"subitem_text_language":"en","subitem_text_value":"Arid Land Research Center, Tottori University / Agricultural Research Corporation (ARC)"},{"subitem_text_language":"en","subitem_text_value":"Department of Crop Science and Biotechnology, Chonbuk National University"},{"subitem_text_language":"en","subitem_text_value":"International Maize and Wheat Improvement Center (CIMMYT)"},{"subitem_text_language":"en","subitem_text_value":"Arid Land Research Center, Tottori University"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"辻本, 壽"},{"creatorName":"ツジモト, ヒサシ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsujimoto, Hisashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Edet, Offiong U.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gorafi, Yasir S. A.","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cho, Seong-woo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishii, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"sr8_s41598-018-34545-x.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensefree":"(C) The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the o","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"sr8_s41598-018-34545-x.pdf","url":"https://repository.lib.tottori-u.ac.jp/record/7371/files/sr8_s41598-018-34545-x.pdf"},"version_id":"6ad38d28-d743-48bd-89df-39e4c4a6f1de"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["12","11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-09-08"},"publish_date":"2022-09-08","publish_status":"0","recid":"7371","relation_version_is_last":true,"title":["Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-09-29T00:48:19.862858+00:00"}