{"created":"2023-08-02T03:58:12.905231+00:00","id":7373,"links":{},"metadata":{"_buckets":{"deposit":"65b6f057-2edc-4830-848a-f02155f7ea49"},"_deposit":{"created_by":10,"id":"7373","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"7373"},"status":"published"},"_oai":{"id":"oai:repository.lib.tottori-u.ac.jp:00007373","sets":["1:9","2:12"]},"author_link":["3618","27439","27440","396","3446","1082","27441","27442","27443","27444","27445","27446","27447","27448","27449","27450"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-07","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"SCIENTIFIC REPORTS"},{"bibliographic_title":"SCIENTIFIC REPORTS","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Gamma-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) is an activatable fluorescent probe that can be activated by γ-glutamyltranspeptidase (GGT). The expression of GGT in the kidney, which is one of the major organs exhibiting enhanced GGT expression, is exclusively localised to the cortex. Here, we aimed to investigate the feasibility of gGlu-HMRG as a probe for the on-site assessment of renal biopsy specimens. gGlu-HMRG fluorescent probe was applied to the renal proximal tubular epithelial cells and cortical collecting duct cells in vitro, mouse kidneys ex vivo, and human biopsy specimens. In addition, the fluorescence intensities in the cortex and the medulla were comparatively evaluated in the biopsy specimens. The fluorescence signal was rapidly detected in the renal proximal tubular epithelial cells, whereas that in the cortical collecting duct cells was not detected. The fluorescence signal was detected in the mouse kidneys ex vivo without markedly affecting the tissue morphology. In the human biopsy specimens, the fluorescence signal in the cortex was significantly distinct from that in the medulla (p?<?0.05). Thus, this fluorescent probe can be used to distinctly identify the renal cortex in the biopsy specimens.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"NATURE PUBLISHING GROUP"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-020-69077-w","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Iyama Takuji, Takata Tomoaki, Yamada Kentaro, et al. A novel method for assessing the renal biopsy specimens using an activatable fluorescent probe. SCIENTIFIC REPORTS. 2020. 10. doi:10.1038/s41598-020-69077-w"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.nature.com/articles/s41598-020-69077-w"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41598-020-69077-w","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the o"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_33":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine / These authors contributed equally: Takuji Iyama and Tomoaki Takata."},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine / These authors contributed equally: Takuji Iyama and Tomoaki Takata."},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine"},{"subitem_text_language":"en","subitem_text_value":"Laboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, The University of Tokyo"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高田, 知朗"},{"creatorName":"タカタ, トモアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Takata, Tomoaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3618","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"100001163","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001163_ja.html"}]},{"creatorNames":[{"creatorName":"ヤマダ, ケンタロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"27439","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"タニグチ, ソウスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"27440","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福田, 佐登子"},{"creatorName":"フクダ, サトコ","creatorNameLang":"ja-Kana"},{"creatorName":"Fukuda, Satoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"396","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"100000933","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100000933_ja.html"}]},{"creatorNames":[{"creatorName":"杉原, 誉明"},{"creatorName":"スギハラ, タカアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Sugihara, Takaaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3446","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60753853","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60753853"},{"nameIdentifier":"100001245","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001245_ja.html"}]},{"creatorNames":[{"creatorName":"磯本, 一"},{"creatorName":"イソモト, ハジメ","creatorNameLang":"ja-Kana"},{"creatorName":"Isomoto, Hajime","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1082","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90322304","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90322304"},{"nameIdentifier":"100001541","nameIdentifierScheme":"研究者総覧鳥取大学","nameIdentifierURI":"http://researchers.adm.tottori-u.ac.jp/html/100001541_ja.html"}]},{"creatorNames":[{"creatorName":"Iyama, Takuji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27441","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Kentaro","creatorNameLang":"en"},{"creatorName":"ヤマダ, ケンタロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"27442","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mae, Yukari","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27443","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Taniguchi, Sosuke","creatorNameLang":"en"},{"creatorName":"タニグチ, 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Yasuteru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27450","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-17"}],"displaytype":"detail","filename":"sr10_s41598-020-69077-w.pdf","filesize":[{"value":"3.8 MB"}],"format":"application/pdf","licensefree":"(C) The Author(s) 2020. 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