| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-08-28 |
| タイトル |
|
|
タイトル |
Exploring Acute Liver Damage: Slimming Health Foods and CYP3A4 Induction |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題 |
acetaminophen |
| キーワード |
|
|
言語 |
en |
|
主題 |
Coleus forskohlii |
| キーワード |
|
|
言語 |
en |
|
主題 |
CYP3A4 induction |
| キーワード |
|
|
言語 |
en |
|
主題 |
DILI |
| キーワード |
|
|
言語 |
en |
|
主題 |
slimming health foods |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
| 著者 |
Adachi,Makiko
Kumagai,Takeshi
法正,恵子
Nagata,Kiyoshi
Fujiyoshi,Masachika
島田,美樹
|
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Pharmacy, Tottori University Hospital |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Medicine and Clinical Science, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Pharmacy, Tottori University Hospital |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Pharmacy, Tottori University Hospital |
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Background: Patients taking multiple drugs and various health foods often develop acute hepatitis. We hypothesized that the interaction between health foods and drug metabolism was the cause of severe liver injury in these patients. Therefore, we studied changes in the activity of the drug-metabolizing enzyme, cytochrome P450 (CYP), using slimming health food extracts and elucidated the molecular mechanism of liver injury onset through hepatotoxicity evaluation. Methods: For cytotoxicity testing, health food extract samples were added to HepG2 cells derived from hepatic parenchymal cells and culture medium, and cell viability was calculated 48 h after culture. To evaluate CYP3A4 induction, 3-1-10 cells constructed with a reporter linked to CYP3A4 gene were used, and reporter activity was measured 48 h after culture. Results: In the chronological order of the slimming health food intake history of the patient, niacinamide and Gymnema sylvestre extracts strongly inhibited HepG2 cell viability. In contrast, dietary supplements A and Coleus forskohlii extract strongly induced CYP3A4 reporter activity. To confirm CYP3A4 induction in humans, humanized CYP3A/pregnane X receptor (PXR) mice were treated with forskolin. CYP3A4 mRNA expression levels were elevated 3.9 times compared to that of the control group (P < 0.05). Conclusion: Coleus forskohlii extract showed the strongest transcriptional activation of CYP3A4 gene. In a mouse model of human-type drug metabolism, forskolin induced CYP3A4 transcription. Thus, we concluded that CYP3A4 induction by Coleus forskohlii is one of the causes of crucial hepatocellular injury, which is a type of liver injury caused by the active metabolite of acetaminophen produced by CYP3A4. |
|
言語 |
en |
| 書誌情報 |
en : Yonago Acta Medica
巻 67,
号 2,
p. 124-134,
ページ数 11,
発行日 2024-05-28
|
| 出版者 |
|
|
出版者 |
Tottori University Medical Press |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
05135710 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
13468049 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00892882 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
(C)2024 Tottori University Medical Press |
| 情報源 |
|
|
|
関連名称 |
Yonago Acta Medica. 2024, 67(2), 124-134. |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam67-2/67-2contents.html |
|
|
関連名称 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam67-2/67-2contents.html |
| 関連サイト |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.33160/yam.2024.05.004 |
|
|
関連名称 |
https://doi.org/10.33160/yam.2024.05.004 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
yam66(4)_124.pdf (1.4 MB)
