| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-10-01 |
| タイトル |
|
|
タイトル |
Development of an AI-Assisted Embryo Selection System Using Iberian Ribbed Newts for Embryo–Fetal Development Toxicity Testing |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題 |
alternative to animal testing |
| キーワード |
|
|
言語 |
en |
|
主題 |
artificial intelligence imaging analysis |
| キーワード |
|
|
言語 |
en |
|
主題 |
embryo–fetal development toxicity testing |
| キーワード |
|
|
言語 |
en |
|
主題 |
Iberian ribbed newt |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
| 著者 |
Saiki,Naofumi
Adachi,Akiko
WEKO
23
| ja |
Adachi,Akiko
kakenhi 鳥取大学
15101
|
| en |
Adachi,Akiko
Tottori University
|
| ja-Kana |
アダチ,アキコ
|
Search repository
Ohnishi,Hiroshi
古賀,敦朗
植木,賢
Kohno,Kiyotaka
Hayashi,Toshinori
大林,徹也
|
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Background: The 3Rs (Reduction, Refinement, Replacement) principle is driving the need for alternative methods in animal testing. Despite advancements in in vitro testing, complex systemic toxicity tests still necessitate in vivo approaches. The aim of this study was to develop a developmental toxicity test protocol using the Iberian ribbed newt (Pleurodeles waltl) as a model organism, integrating AI image analysis for embryo selection to improve test accuracy and reproducibility. Methods: We established a developmental toxicity test protocol based on the zebrafish test. Gonadotropin was administered to induce ovulation, and in vitro fertilization was performed. Embryos were imaged at 5–6 and 6–7 h post-fertilization. AI image analysis was utilized to assess embryo viability. The test chemical was administered 24–48 h post-fertilization, and morphological changes were observed daily until day 8. Additionally, a time-lapse photography system was constructed to monitor embryonic development. Results: Out of 24 cultured embryos, 75% developed normally to the late tail bud stage or initial hatching stage, whereas 25% experienced developmental arrest or death. AI image analysis achieved high accuracy in classifying embryos, with overall accuracies of 92.0% and 92.9% for two learning models. The AI system demonstrated higher precision in the selection of viable embryos compared to visual inspection. Conclusion: The Iberian ribbed newt presents a viable alternative model for developmental toxicity testing, adhering to the 3Rs principles. The integration of AI image analysis substantially enhances the accuracy and reproducibility of embryo selection, providing a reliable method for evaluating developmental toxicity in pharmaceuticals. |
|
言語 |
en |
| 書誌情報 |
en : Yonago Acta Medica
巻 67,
号 3,
p. 233-241,
ページ数 9,
発行日 2024-08-26
|
| 出版者 |
|
|
出版者 |
Tottori University Medical Press |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
05135710 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
13468049 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00892882 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
(C)2024 Tottori University Medical Press |
| 情報源 |
|
|
|
関連名称 |
Yonago Acta Medica. 2024, 67(3), 233-241. |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam67-3/67-3contents.html |
|
|
関連名称 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam67-3/67-3contents.html |
| 関連サイト |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.33160/yam.2024.08.011 |
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|
関連名称 |
https://doi.org/10.33160/yam.2024.08.011 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
yam67(3)_233.pdf (2.7 MB)
