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Imaging of Liver Tissues Using a Combination of Silver Impregnation and Low-Vacuum Scanning Electron Microscopy; A Simple Method for the High-Resolution Visualization of Reticulin Structures with Applicability to a Quantitative Analysis
https://repository.lib.tottori-u.ac.jp/records/4611
https://repository.lib.tottori-u.ac.jp/records/461131926ad7-e6ae-4e08-8f95-81e68e6b79d0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
yam66(1)_78.pdf (3.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-02-28 | |||||
| タイトル | ||||||
| タイトル | Imaging of Liver Tissues Using a Combination of Silver Impregnation and Low-Vacuum Scanning Electron Microscopy; A Simple Method for the High-Resolution Visualization of Reticulin Structures with Applicability to a Quantitative Analysis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | carcinoma | |||||
| キーワード | ||||||
| 主題 | hepatocellular | |||||
| キーワード | ||||||
| 主題 | liver diseases | |||||
| キーワード | ||||||
| 主題 | microscopy | |||||
| キーワード | ||||||
| 主題 | electron | |||||
| キーワード | ||||||
| 主題 | scanning | |||||
| キーワード | ||||||
| 主題 | image processing | |||||
| キーワード | ||||||
| 主題 | computer-assisted | |||||
| キーワード | ||||||
| 主題 | reticulin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | carcinoma | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | hepatocellular | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | liver diseases | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | microscopy | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | electron | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | scanning | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | image processing | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | computer-assisted | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | reticulin | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
桑本, 聡史
× 桑本, 聡史× 長田, 佳子× 松下, 倫子× 海藤, 俊行× 梅北, 善久× 林, 一彦× 北村, 幸郷× Izuta, Mikihiro× Kamiya, Tetsutaro× Inaga, Sumire |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Pharmacology, Department of Pathophysiological and Therapeutic Sciences, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Anatomy, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Anatomy, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Background: Low-vacuum scanning electron microscopy (LVSEM) enables the detailed three-dimensional imaging of archival tissues without special pretreatments. The clinical utility of LVSEM in the assessment of liver diseases has not yet been defined. So, we investigated the utility of LVSEM imaging in morphological assessments of normal and diseased liver tissues, with a focus on reticulin structures. Methods: Formalin-fixed tissue samples of two normal livers and two hepatocellular carcinomas with background regenerative nodules/areas were stained with platinum blue stain or silver-impregnated using Watanabe’s method and then comparatively observed under LVSEM. We also evaluated the applicability of LVSEM imaging of liver tissues to a quantitative analysis using a digital image analysis technique. Results: Optimal high-resolution images of reticulin structures were obtained using 10-μm-thick silver-impregnated sections. Reticulin fibers were clearly observed to run dendritically around sinusoids in normal livers, and markedly increased in regenerative nodules/areas. Normal reticulin frameworks were lost in hepatocellular carcinoma, leaving a few fragments of reticulin fibers within tumors. Moreover, when a quantitative analysis was applied to these images, we successfully demonstrated a significantly higher reticulin fiber density in regenerative nodules/areas than in the normal liver (P < 0.05). Conclusion: We not only obtained detailed three-dimensional images of reticulin structures in various liver tissues by LVSEM combined with silver impregnation but also showed their applicability to a quantitative analysis. The method presented herein may be applied to future studies for the more accurate diagnosis and better classification/risk stratification of various liver diseases. | |||||
| 書誌情報 |
Yonago Acta Medica en : Yonago Acta Medica 巻 66, 号 1, p. 78-86, 発行日 2023-02-20 |
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| 出版者 | ||||||
| 出版者 | Tottori University Medical Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05135710 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.33160/yam.2023.02.009 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2023 Tottori University Medical Press. | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta Medica. 2023, 66(1), 78-86. doi:10.33160/yam.2023.02.009 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
| 関連名称 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.33160/yam.2023.02.009 | |||||
| 関連名称 | https://doi.org/10.33160/yam.2023.02.009 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| EISSN | ||||||
| 値 | 13468049 | |||||
