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Resolvin E1 Inhibits Osteoclastogenesis and Bone Resorption by Suppressing IL-17-induced RANKL Expression in Osteoblasts and RANKL-induced Osteoclast Differentiation
https://repository.lib.tottori-u.ac.jp/records/4807
https://repository.lib.tottori-u.ac.jp/records/48074d6ca2bd-f482-42be-8e88-6df95b723b8d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
yam61(1)_8.pdf (2.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | Resolvin E1 Inhibits Osteoclastogenesis and Bone Resorption by Suppressing IL-17-induced RANKL Expression in Osteoblasts and RANKL-induced Osteoclast Differentiation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | interleukin-17 | |||||
| キーワード | ||||||
| 主題 | osteoblasts | |||||
| キーワード | ||||||
| 主題 | osteoclasts | |||||
| キーワード | ||||||
| 主題 | RANK ligand | |||||
| キーワード | ||||||
| 主題 | resolvin E1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | interleukin-17 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | osteoblasts | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | osteoclasts | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | RANK ligand | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | resolvin E1 | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Funaki, Yoshihiro
× Funaki, Yoshihiro× Hasegawa, Yasuyuki× Okazaki, Ryota× Yamasaki, Akira× Sueda, Yuriko× Yamamoto, Akihiro× Yanai, Masaaki× Fukushima, Takehito× Harada, Tomoya× Makino, Haruhiko× Shimizu, Eiji× Hasegawa, Yasuyuki× Sueda, Yuriko× Yanai, Masaaki× Fukushima, Takehito |
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| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 著者所属 | ||||||
| 値 | Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, School of Medicine, | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 【Background】 Resolvin E1 (RvE1) derived from the ω-3 polyunsaturated fatty acid eicosapentaenoic acid is known to be a potent pro-resolving lipid mediator that prevents chronic inflammation and osteoclastogenesis. We investigated the inhibitory effects of RvE1 on osteoclastogenesis and bone resorption to clarify its therapeutic potential for rheumatoid arthritis (RA). 【Methods】 Receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation was assessed with tartrate-resistant acid phosphatase staining. RANKL-induced bone resorption was assessed by the measurement of pit formation using calcium phosphate-labeled fluorescent polyanionic molecules in RAW264.7 cells as osteoclast precursors. The effects of RvE1 on the RANKL-induced mRNA expression of osteoclast-specific genes and transcriptional factors such as c-fos and nuclear factor of activated T cells c1 (NFATc1) in RAW264.7 cells were measured by quantitative real-time PCR. The distribution of NFATc1 induced by RANKL was evaluated by immunofluorescence staining in RAW264.7 cells. To analyze the mechanism of the inhibitory effect of RvE1 on osteoclastogenesis, we measured IL-17-induced RANKL mRNA expression in MC3T3-E1 osteoblast cells treated with RvE1 using quantitative real-time PCR and determined the level of prostaglandin E2 (PGE2) production by enzyme-linked immunosorbent assay. 【Results】 RvE1 significantly suppressed RANKL-induced osteoclast differentiation and bone resorption. RvE1 inhibited the RANKL-induced mRNA expression of osteoclast-specific genes along with the transcription factors NFATc1 and c-fos. Moreover, NFATc1 translocation from the cytoplasm to the nucleus of RAW264.7 cells was suppressed following RvE1 treatment. RvE1 also inhibited IL-17-induced RANKL mRNA expression and PGE2 production in MC3T3-E1 cells. 【Conclusion】 RvE1 inhibited osteoclastogenesis and bone resorption by suppressing RANKL-induced NFATc1 and c-fos expression in osteoclasts and IL-17-induced RANKL expression through the autocrine action of PGE2 in osteoblasts. Our data suggest RvE1 as a new therapeutic target of RA. | |||||
| 書誌情報 |
Yonago Acta Medica en : Yonago Acta Medica 巻 61, 号 1, p. 8-18, 発行日 2018-03-28 |
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| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05135710 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.33160/yam.2018.03.002 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta Medica. 2018, 61(1), 8-18 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.lib.tottori-u.ac.jp/yam/yam/yam61-1/61-1contents.html | |||||
| 関連名称 | http://www.lib.tottori-u.ac.jp/yam/yam/yam61-1/61-1contents.html | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| EISSN | ||||||
| 値 | 1346-8049 | |||||
