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The PI3K-Akt Pathway in SN-38-Induced Apoptosis in Human Gastric Cancer Cell Lines
https://repository.lib.tottori-u.ac.jp/records/5076
https://repository.lib.tottori-u.ac.jp/records/50762a4ca1c5-a1f0-4892-8a2c-740aa941da70
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
48_007-016.pdf (440.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | The PI3K-Akt Pathway in SN-38-Induced Apoptosis in Human Gastric Cancer Cell Lines | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 主題 | gastric cancer | |||||
| キーワード | ||||||
| 主題 | PI3K-Akt pathway | |||||
| キーワード | ||||||
| 主題 | SN-38 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | gastric cancer | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | PI3K-Akt pathway | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | SN-38 | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Yoshioka, Shizue
× Yoshioka, Shizue× Tatebe, Shigeru× Tokuyasu, Naruo× Taniguchi, Kenjiro× Ogami, Yoshimi× Yamamoto, Osamu× Ashida, Keigo× Gomyo, Yoshihito× Kondo, Akira× Tsujitani, Shunichi× Ikeguchi, Masahide× Yoshioka, Shizue× Tatebe, Shigeru× Taniguchi, Kenjiro× Ogami, Yoshimi× Yamamoto, Osamu× Gomyo, Yoshihito× Tsujitani, Shunichi |
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| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | SN-38, an active metabolite of a topoisomerase I inhibitor, CPT-11, exhibits a cytotoxic effect by inducing apoptosis in cancer cells. Phosphatidylinositol-3-OH kinase (PI3K)-Akt signaling is known to protect a variety of cells from apoptosis. The relationship between resistance to SN-38-induced apoptosis and the PI3K-Akt pathway in human gastric cancer cells is unknown. Here, we did an investigation using two gastric cancer cell lines, MKN1 and MKN45. Cell viability was determined by sodium 3'-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate (XTT) assay. Apoptosis was confirmed by fluorescence microscopy using Hoechst 33342 staining. Expression levels of phospho-Akt (pAkt) were determined by Western blotting. After being treated with SN-38, the populations of sub-G1 cells were induced by flow cytometry in 36.8% of MKN45 cells more frequently than in 13.5% of MKN1 cells. SN-38 inhibited the expression of pAkt dose-dependently in MKN45 cells, but not in MKN1 cells. In MKN1 cells, an additional pretreatment with the PI3K inhibitor, LY294002, led to the inhibition of pAkt expression and induced apoptosis. The results suggested that SN-38 induces apoptosis by decreasing PI3K-Akt survival signaling, the anti-apoptotic signals, in human gastric cancer cells. Akt inhibitor might be a useful anti-tumor agent in combination with CPT-11. | |||||
| 書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 48, 号 1, p. 7-16, 発行日 2005-03 |
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| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13468049 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta medica. 2005, 48(1), 7-16 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
