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Induction of Apoptosis of Rat Neonatal Cardiomyocytes by Chemical Ischemia and Reoxygenation: The Role of Phosphatidylserine
https://repository.lib.tottori-u.ac.jp/records/5151
https://repository.lib.tottori-u.ac.jp/records/5151246d98d3-f8c3-492e-ade1-e40ba9631991
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
43_011-018.pdf (232.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | Induction of Apoptosis of Rat Neonatal Cardiomyocytes by Chemical Ischemia and Reoxygenation: The Role of Phosphatidylserine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | annexin V | |||||
| キーワード | ||||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 主題 | cardiomyocyte | |||||
| キーワード | ||||||
| 主題 | phosphatidylserine | |||||
| キーワード | ||||||
| 主題 | reoxygenation injury | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | annexin V | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | cardiomyocyte | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | phosphatidylserine | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | reoxygenation injury | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Ono, Kimiyo
× Ono, Kimiyo× Ishiguro, Shingo× Kuroda, Hiroaki× Ohgi, Shigetsugu× Ono, Kimiyo× Ishiguro, Shingo× Kuroda, Hiroaki |
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| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Ischemia/reperfusion injury plays a crucial role in the induction of the cell death of myocytes. The precise mechanism of the cell death, however, has not been elucidated enough. This study examined the cell death of rat neonatal myocytes induced by chemical ischemia and reoxygenation with an in vitro model, in terms of apoptosis, and the role of phosphatidylserine, which is recognized with annexin V. Chemical ischemia and reoxygenation were conducted on the cultured myocytes obtained from 1- or 2-day-old Wistar rats. The cells were divided into 4 groups exposed to chemical ischemia for 9 h (Group A), 18 h (Group B) and 24 h (Group C) and one group not exposed to chemical ischemia (Control Group). DNA ladder formation on agarose gel electrophoresis was noted in Groups B and C followed by reoxygenation, but not in Group A, as well as all 4 groups without reoxygenation. There were cells positive to terminal deoxynucleotidyl transferase-mediated dUDP-biotin nick end labeling in all 3 groups except for the Control Group; after reoxygenation, the number of cells became larger in Groups B and C than in Group A. Flow cytometry revealed that annexin V-positive cells were 1.15 ± 0.82% in the Control Group, 4.07 ± 3.8% in Group A without reoxygenation and 15.5 ± 6.3% in Group A after 30-min reoxygenation, respectively; the value was significantly higher in the latter than the former two (P < 0.01). Although 18-h and 24-h ischemia increased the annexin V-positive cells, reoxygenation did not alter the number of cells in Groups B and C. These results indicate that i) chemical ischemia followed by reoxygenation variably induces apoptosis of rat myocytes, ii) long-term ischemia causes phosphatidylserine translocation on the cell surface membrane, regardless of reoxygenation and iii) mild ischemia necessitates reoxygenation to translocate phosphatidylserine, which might play a crucial role in the initiation of apoptosis of the myocytes. | |||||
| 書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 43, 号 1, p. 11-18, 発行日 2000-03 |
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| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13468049 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta medica. 2000, 43(1), 11-18 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
