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  1. 学部学科区分一覧
  2. 医学部・医学系研究科・医学部附属病院
  1. 資料タイプ一覧
  2. 学術雑誌論文
  1. 鳥取大学の刊行物
  2. Yonago Acta Medica
  3. 43
  4. 1

Induction of Apoptosis of Rat Neonatal Cardiomyocytes by Chemical Ischemia and Reoxygenation: The Role of Phosphatidylserine

https://repository.lib.tottori-u.ac.jp/records/5151
https://repository.lib.tottori-u.ac.jp/records/5151
246d98d3-f8c3-492e-ade1-e40ba9631991
名前 / ファイル ライセンス アクション
43_011-018.pdf 43_011-018.pdf (232.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-06-22
タイトル
タイトル Induction of Apoptosis of Rat Neonatal Cardiomyocytes by Chemical Ischemia and Reoxygenation: The Role of Phosphatidylserine
言語 en
言語
言語 eng
キーワード
主題 annexin V
キーワード
主題 apoptosis
キーワード
主題 cardiomyocyte
キーワード
主題 phosphatidylserine
キーワード
主題 reoxygenation injury
キーワード
言語 en
主題 annexin V
キーワード
言語 en
主題 apoptosis
キーワード
言語 en
主題 cardiomyocyte
キーワード
言語 en
主題 phosphatidylserine
キーワード
言語 en
主題 reoxygenation injury
資源タイプ
資源タイプ journal article
著者 Ono, Kimiyo

× Ono, Kimiyo

WEKO 19120

Ono, Kimiyo

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Ishiguro, Shingo

× Ishiguro, Shingo

WEKO 19121

Ishiguro, Shingo

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Kuroda, Hiroaki

× Kuroda, Hiroaki

WEKO 19122

Kuroda, Hiroaki

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Ohgi, Shigetsugu

× Ohgi, Shigetsugu

WEKO 2779
e-Rad 50168845

Ohgi, Shigetsugu

en Ohgi, Shigetsugu

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Ono, Kimiyo

× Ono, Kimiyo

WEKO 19123

en Ono, Kimiyo

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Ishiguro, Shingo

× Ishiguro, Shingo

WEKO 19124

en Ishiguro, Shingo

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Kuroda, Hiroaki

× Kuroda, Hiroaki

WEKO 19125

en Kuroda, Hiroaki

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著者所属
値 Second Department of Surgery, Faculty of Medicine, Tottori University
著者所属
値 Second Department of Surgery, Faculty of Medicine, Tottori University
著者所属
値 Second Department of Surgery, Faculty of Medicine, Tottori University
著者所属
値 Second Department of Surgery, Faculty of Medicine, Tottori University
抄録
内容記述タイプ Other
内容記述 Ischemia/reperfusion injury plays a crucial role in the induction of the cell death of myocytes. The precise mechanism of the cell death, however, has not been elucidated enough. This study examined the cell death of rat neonatal myocytes induced by chemical ischemia and reoxygenation with an in vitro model, in terms of apoptosis, and the role of phosphatidylserine, which is recognized with annexin V. Chemical ischemia and reoxygenation were conducted on the cultured myocytes obtained from 1- or 2-day-old Wistar rats. The cells were divided into 4 groups exposed to chemical ischemia for 9 h (Group A), 18 h (Group B) and 24 h (Group C) and one group not exposed to chemical ischemia (Control Group). DNA ladder formation on agarose gel electrophoresis was noted in Groups B and C followed by reoxygenation, but not in Group A, as well as all 4 groups without reoxygenation. There were cells positive to terminal deoxynucleotidyl transferase-mediated dUDP-biotin nick end labeling in all 3 groups except for the Control Group; after reoxygenation, the number of cells became larger in Groups B and C than in Group A. Flow cytometry revealed that annexin V-positive cells were 1.15 ± 0.82% in the Control Group, 4.07 ± 3.8% in Group A without reoxygenation and 15.5 ± 6.3% in Group A after 30-min reoxygenation, respectively; the value was significantly higher in the latter than the former two (P < 0.01). Although 18-h and 24-h ischemia increased the annexin V-positive cells, reoxygenation did not alter the number of cells in Groups B and C. These results indicate that i) chemical ischemia followed by reoxygenation variably induces apoptosis of rat myocytes, ii) long-term ischemia causes phosphatidylserine translocation on the cell surface membrane, regardless of reoxygenation and iii) mild ischemia necessitates reoxygenation to translocate phosphatidylserine, which might play a crucial role in the initiation of apoptosis of the myocytes.
書誌情報 Yonago Acta medica
en : Yonago Acta medica

巻 43, 号 1, p. 11-18, 発行日 2000-03
出版者
出版者 Tottori University Faculty of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 13468049
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00892882
権利
権利情報 Yonago Acta medica 編集委員会
情報源
関連名称 Yonago Acta medica. 2000, 43(1), 11-18
著者版フラグ
出版タイプ VoR
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