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Biochemical Functions and mRNA Expression of Rat Hepatocytes in a Bioartificial Liver Module
https://repository.lib.tottori-u.ac.jp/records/5177
https://repository.lib.tottori-u.ac.jp/records/5177d1e87483-e362-46a9-a164-891826a07422
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
42_103-112.pdf (57.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | Biochemical Functions and mRNA Expression of Rat Hepatocytes in a Bioartificial Liver Module | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | albumin mRNA | |||||
| キーワード | ||||||
| 主題 | bioartificial liver | |||||
| キーワード | ||||||
| 主題 | cytochrome P450 2B1 mRNA | |||||
| キーワード | ||||||
| 主題 | hepatic function | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | albumin mRNA | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | bioartificial liver | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | cytochrome P450 2B1 mRNA | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | hepatic function | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Okada, Shinichi
× Okada, Shinichi× Kono, Yumi× Shiraki, Kazuo× Kono, Yumi |
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| 著者所属 | ||||||
| 値 | Department of Pediatrics, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Department of Pediatrics, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Department of Pediatrics, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Evaluation of hepatic functions of the bioartificial liver (BAL) module is essential for developing an optimal BAL system which can be applied to a patient with fulminant hepatic failure. Although hepatic function in BALs has been evaluated only in biochemical activities in previous reports, we assessed a newly designed BAL from a hollow fiber type not only in biochemical activities such as gluconeogenesis, ureogenesis and albumin synthesis but also in the expressions of albumin messenger RNA (mRNA) and cytochrome P450 2B1 mRNA by Northern blot analysis. One to 3 × 108 hepatocytes isolated from adult rats were suspended in hydrated collagen, loaded into a hollow fiber module and perfused with L-15 medium supplemented with various hormones for 2 to 13 days. Both production rates of glucose and urea nitrogen were highest on day 1 (610 ?g glucose/107 cells/h and 76.1 ?g urea nitrogen/107 cells/h, respectively). Ureogenesis was maintained for 8 days in BAL, but gluconeogenesis decreased after 7 days. Albumin production rate increased to day 3, reached its maximum (11.0 ?g/107 cells/h) and then gradually decreased. The expression of albumin mRNA was highest on day 2 and detectable until day 11. Cytochrome P450 2B1 mRNA was detected only on day 2. Northern blot analysis showed that the maintenance period in each hepatic function was different. These results suggested that assessment of the functions at a molecular level was invaluable in the development of a potential BAL for clinical use. It might be necessary to reconstruct the lobular system as well as a 3-dimensional environment for hepatocytes in the BAL to maintain the ability of detoxification such as cytochrome P450s. | |||||
| 書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 42, 号 1, p. 103-112, 発行日 1999-03 |
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| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13468049 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta medica. 1999, 42(1), 103-112 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
